{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:13:26Z","timestamp":1790176406468,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,8,2]],"date-time":"2013-08-02T00:00:00Z","timestamp":1375401600000},"content-version":"vor","delay-in-days":32,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Deletion 13q14.3 is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL). Previously it was reported that miR\u201015\/16 is the target of 13q14 deletions and plays a tumor suppressor role by suppressing Bcl\u20102. Therefore, Bcl\u20102 expression was examined more closely to determine whether it would predict 13q14 deletion status.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A multi\u2010color flow panel consisting of anti\u2010Bcl\u20102\/anti\u2010lambda\/anti\u2010kappa\/CD19\/CD5\/CD3\/CD20 was performed. The ability of Bcl\u20102 to predict 13q14 deletion was tested using the conventional Bcl\u20102 index (c\u2010index): mean fluorescence intensity (MFI) of CLL clone\/MFI of residual T cells. Fifty\u2010four untreated CLL\/MBL patients were studied. Bimodal Bcl\u20102 expression was evaluated to test the ability of Bcl\u20102 to detect intraclonal heterogeneity. Other CLL prognostic markers including CD38, CD49d, CD26, and CD69 were evaluated. FISH was performed on selected sorted populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Bcl\u20102 c\u2010index strongly predicts del13q14 <jats:italic>P<\/jats:italic> &lt; 0.0001. A statistically significant association was observed between the percentage of cells carrying the deletion and the level of Bcl\u20102 expression <jats:italic>P<\/jats:italic> &lt; 0.05. Cells sorted based on Bcl\u20102 expression showed enrichment of both hemizygous and homozygous del 13q14 cells. Also, we observed that an alteration in Bcl\u20102 level over time predicts changes in 13q14 deletion status. And a statistically significant correlation between the bimodal pattern of CD69 expression and the presence of 13q14 deletion was found <jats:italic>P<\/jats:italic> &lt; 0.0001.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Bcl\u20102 expression using the c\u2010index strongly predicts 13q14 deletion and can be used to distinguish homozygous, heterozygous, and diploid CLL clonal cells. Further systematic studies of this biomarker are needed for confirmation and expansion of these findings. Published 2013 Wiley\u2010Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21090","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T16:21:03Z","timestamp":1368548463000},"page":"237-247","source":"Crossref","is-referenced-by-count":16,"title":["Bcl\u20102 level as a biomarker for 13q14 deletion in CLL"],"prefix":"10.1002","volume":"84B","author":[{"given":"Heba A.","family":"Degheidy","sequence":"first","affiliation":[{"name":"Center for Biologics Evaluation and Research FDA Bethesda Maryland"},{"name":"Center for Devices and Radiological Health Silver Spring Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahinaz M.","family":"Gadalla","sequence":"additional","affiliation":[{"name":"Clinical Genetics Branch Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Z. 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