{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:16:20Z","timestamp":1790810180392,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T00:00:00Z","timestamp":1439856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002704","name":"Fundaci\u00f3n Cient\u00edfica Asociaci\u00f3n Espa\u00f1ola Contra el C\u00e1ncer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002704","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cooperative Cancer Cancer Research Thematic Network (RTICC); Instituto de Salud Carlos III-FEDER, Ministerio de Econom\u00c3\u00ada y Competitividad, Madrid, Spain","award":["RD12\/0036\/0048"],"award-info":[{"award-number":["RD12\/0036\/0048"]}]},{"name":"Fundaci\u00c3\u00b3n Samuel Solorzano Barruso (University of Salamanca)"},{"name":"Gerencia Regional de Salud de Castilla y Le\u00c3\u00b3n (SACYL)","award":["BIO\/SA24\/13"],"award-info":[{"award-number":["BIO\/SA24\/13"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>Despite diagnostic criteria are currently available for the distinct subtypes of monocytic\u2010lineage neoplasias, a number of partially overlapping features still remain evident, which may hamper their differential diagnosis. An accurate identification and characterization of monocytic cells is of major relevance for the diagnosis and classification of these neoplasias. In this regard, as compared to other conventional techniques, flow cytometry has shown the highest sensitivity for detection of early monocytic commitment of (normal and neoplastic) bone marrow CD34<jats:sup>+<\/jats:sup> hematopoietic precursors as well as of monocytic aberrations and maturation blockades, which are frequently associated with clonal myeloid disorders.<\/jats:p><jats:p>In the present paper we provide basic principles and criteria for multiparameter flow cytometry identification and characterization of bone marrow monocytic cells that contribute to an improved diagnostic and classification of monocytic lineage\u2010associated acute leukemias in clinical settings, particularly when using the EuroFlow antibody panels. \u00a9 2015 International Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.21219","type":"journal-article","created":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T10:04:27Z","timestamp":1439892267000},"page":"218-227","source":"Crossref","is-referenced-by-count":69,"title":["Introduction to the diagnosis and classification of monocytic\u2010lineage leukemias by flow cytometry"],"prefix":"10.1002","volume":"92","author":[{"given":"Sergio","family":"Matarraz","sequence":"first","affiliation":[{"name":"Servicio De Citometr\u00eda (NUCLEUS) and Department of Medicine Centro De Investigaci\u00f3n Del C\u00e1ncer (IBMCC, University of Salamanca\u2010CSIC) and IBSAL Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Almeida","sequence":"additional","affiliation":[{"name":"Servicio De Citometr\u00eda (NUCLEUS) and Department of Medicine Centro De Investigaci\u00f3n Del C\u00e1ncer (IBMCC, University of Salamanca\u2010CSIC) and IBSAL Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Flores\u2010Montero","sequence":"additional","affiliation":[{"name":"Servicio De Citometr\u00eda (NUCLEUS) and Department of Medicine Centro De Investigaci\u00f3n Del C\u00e1ncer (IBMCC, University of Salamanca\u2010CSIC) and IBSAL Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quentin","family":"L\u00e9crevisse","sequence":"additional","affiliation":[{"name":"Servicio De Citometr\u00eda (NUCLEUS) and Department of Medicine Centro De Investigaci\u00f3n Del C\u00e1ncer (IBMCC, University of Salamanca\u2010CSIC) and IBSAL Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Guerri","sequence":"additional","affiliation":[{"name":"Servicio De Citometr\u00eda (NUCLEUS) and Department of Medicine Centro De Investigaci\u00f3n Del C\u00e1ncer (IBMCC, University of Salamanca\u2010CSIC) and IBSAL Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Servicio De Citometr\u00eda (NUCLEUS) and Department of Medicine Centro De Investigaci\u00f3n Del C\u00e1ncer (IBMCC, University of Salamanca\u2010CSIC) and IBSAL Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"B\u00e1rrena","sequence":"additional","affiliation":[{"name":"Servicio De Citometr\u00eda (NUCLEUS) and Department of Medicine Centro De Investigaci\u00f3n Del C\u00e1ncer (IBMCC, University of Salamanca\u2010CSIC) and IBSAL Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent H. 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