{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T15:36:35Z","timestamp":1726760195050},"reference-count":5,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T00:00:00Z","timestamp":1433203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Double\u2010hit lymphoma (DHL) is a rare type of lymphoma with concurrent chromosomal translocations of C\u2010MYC with BCL2 or BCL6, associated with unfavorable prognosis. We describe a case of DHL in a 79\u2010year\u2010old female patient previously diagnosed with diffuse large B\u2010cell lymphoma (DLBCL) with an early relapse in the ascitic fluid. A seven\u2010color multiparametric flow cytometry immunophenotyping study of the ascitic fluid was carried out, and revealed 99.78% of large in size and high cellular complexity B\u2010cells positive for CD19, CD10 (64.27%), CD45 dim, CD22 dim, CD25 (60%), CD43 bright, CD38 bright, and IgM (18.53%); and negative for CD20, CD5, CD23, CD79b, CD103, CD200, CD11c, and FMC7, and 78.99% without light chain expression and 21% with Lambda chain restriction. Due to the expression of CD19 and CD10 with overexpression of BCL\u20102 protein and due to CD43 and CD38 positivity detected, those cells showed features between DLBCL and Burkitt lymphoma. Fluorescence in situ hybridization (FISH) confirmed both c\u2010MYC\/IGH and BCL2\/IGH rearrangement. Our findings may help to identify cases requiring additional cytogenetic analysis. \u00a9 2015 International Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.21227","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T21:47:27Z","timestamp":1421963247000},"page":"543-545","source":"Crossref","is-referenced-by-count":8,"title":["The utility of multiparametric seven\u2010color flow cytometry in the detection of double hit lymphoma in ascitic fluid samples"],"prefix":"10.1002","volume":"90","author":[{"given":"Raul","family":"Cordoba","sequence":"first","affiliation":[{"name":"Lymphoma Unit Hematology Deparment Fundacion Jimenez Diaz University Hospital, Health Research Institute IIS\u2010FJD Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Central Laboratory, Flow Cytometry Unit, Infanta Sofia University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Masso","sequence":"additional","affiliation":[{"name":"Hematology Department Infanta Sof\u00eda University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Ataulfo","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Central Laboratory, Hematology Unit, Infanta Sofia University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luz","family":"Conejo","sequence":"additional","affiliation":[{"name":"Central Laboratory, Flow Cytometry Unit, Infanta Sofia University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Velasco","sequence":"additional","affiliation":[{"name":"Central Laboratory, Hematology Unit, Infanta Sofia University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan\u2010Manuel","family":"Alonso","sequence":"additional","affiliation":[{"name":"Central Laboratory, Hematology Unit, Infanta Sofia University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus","family":"Villarrubia","sequence":"additional","affiliation":[{"name":"Central Laboratory, Hematology Unit, Infanta Sofia University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Cava","sequence":"additional","affiliation":[{"name":"Central Laboratory, Hematology Unit, Infanta Sofia University Hospital San Sebastian De Los Reyes Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Llamas","sequence":"additional","affiliation":[{"name":"Lymphoma Unit Hematology Deparment Fundacion Jimenez Diaz University Hospital, Health Research Institute IIS\u2010FJD Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.41.4342"},{"key":"e_1_2_5_3_1","first-page":"788","article-title":"Double\u2010hit and triple\u2010hit lymphomas arising from follicular lymphoma following acquisition of MYC: Report of two cases and literature review","volume":"6","author":"Xu X","year":"2013","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21076"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","unstructured":"WuD WoodBL DorerR FrommJR.Utility of flow cytometry for immunophenotyping double\u2010hit lymphomas.Cytometry B Clin Cytom2013;84:398","DOI":"10.1002\/cyto.b.21117"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP7YLDTJPLCE5F"}],"container-title":["Cytometry Part B: 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