{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:29:09Z","timestamp":1790666949988,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,4,7]],"date-time":"2015-04-07T00:00:00Z","timestamp":1428364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Analysis for 24 variable regions of the T\u2010cell\u2010receptor beta chain by flow cytometry (Vbeta) is a new technique to detect clonal alpha\u2010beta T lymphocytes and is characteristically performed on peripheral blood. Angioimmunoblastic T\u2010cell lymphoma (AITL) has increased neoplastic follicular\u2010helper T cells (FHT), which often express CD10; but nonneoplastic, CD10\u2010positive T cells may be associated with reactive lymphadenopathy and with B\u2010cell lymphomas. This study documents the utility of Vbeta analysis of FHT in specimens of AITL from blood, from marrow, and from lymph nodes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The electronic medical record in the flow cytometry laboratory was searched for specimens that were analyzed by flow cytometry for Vbeta and that were involved by AITL. Flow cytometry was performed for the following antigens: T\u2010cell\u2010associated proteins, CD10, CD56, CD94, CD161, killer\u2010cell immunoglobulin\u2010like receptors, alpha\u2010beta T\u2010cell receptor, gamma\u2010delta T\u2010cell receptor, and Vbeta.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five patients had six specimens of blood (two), of bone marrow (one), or of lymph nodes (three). Immunophenotypic aberrances were detected for antigens: CD2 (2\/6), CD3 (6\/6), CD4 (1\/6), CD5 (1\/6), CD7 (5\/6), and CD45 (2\/6). All abnormal T\u2010cell populations expressed CD4, and most expressed CD10 (5\/6). Four specimens were clonally restricted for Vbeta. Two specimens lacked the alpha\u2010beta T\u2010cell receptor and Vbeta.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Vbeta analysis by flow cytometry can be used to detect clonal alpha\u2010beta FHT in AITL, which may be difficult to diagnose with early involvement. Abnormal Vbeta expression on CD10\u2010positive T cells confirms that FHT are the neoplastic cells. \u00a9 2014 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21229","type":"journal-article","created":{"date-parts":[[2015,1,24]],"date-time":"2015-01-24T00:11:41Z","timestamp":1422058301000},"page":"190-193","source":"Crossref","is-referenced-by-count":4,"title":["Abnormal immunophenotype of the T\u2010cell\u2010receptor beta Chain in follicular\u2010helper T cells of angioimmunoblastic T\u2010cell lymphoma"],"prefix":"10.1002","volume":"88","author":[{"given":"Z. Jenny","family":"Mao","sequence":"first","affiliation":[{"name":"Hematopathology Laboratory, University of Minnesota Medical Center Fairview Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Surowiecka","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Linden","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P.","family":"Singleton","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.1.165"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPV2D1DDSGJDBW"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPPT93VZMAREHK"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.74"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.08003.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MP.0000084630.64243.D1"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/MC7QRGPTV0LRR98X"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20000615)42:3<180::AID-CYTO4>3.0.CO;2-0"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20107"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2007.02195.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1309\/28YP0DELGKEJGRXG"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04201.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2005.05.008"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404013"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.53"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.2.627"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21483"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21229","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21229","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T23:41:03Z","timestamp":1696635663000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,7]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1002\/cyto.b.21229"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21229","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,7]]}}}