{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:54:05Z","timestamp":1789894445610,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T00:00:00Z","timestamp":1430352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2015,7,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>We have designed one\u2010tube 14\u2010antibody 10\u2010color flow cytometry (FCM) panel that would provide maximum information on lymphoid and myeloid cell subsets in bone marrow aspirates (BMA) from patients with cytopenia(s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Samples and Methods<\/jats:title><jats:p>BMA from 8 normal donors, from 286 non\u2010malignant hospital controls, 92 myelodysplastic syndromes (MDS), 47 myeloproliferative neoplasms (MPN), and from 14 MDS\/MPN patients were investigated. One tube 14\u2010monoclonal antibody (MAb) 10\u2010fluorochrome panel included: kappa+CD4 FITC, Lambda+CD8 PE, CD3\u2009+\u2009CD14 ECD, CD34 APC, CD20+CD56 PC7, CD10 APC\u2010A750, CD19 APC\u2010A700, CD33 PC5.5, CD5 PB, and CD45 KO. Kappa\/lambda expression was evaluated separately in CD19+, CD10+ and CD5+ B\u2010cells. CD4+CD3+, CD8+CD3+, CD5+CD3+ T\u2010lymphocyte subsets were enumerated. Blasts were evaluated using CD45\/SSC and CD34 gating. The FCM score for MDS (sc. Ogata score) included CD34+ myeloblast and B\u2010progenitor cluster size, myeloblast\/lymphocyte CD45 expression, and granulocyte\/lymphocyte SSC ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Abnormal lymphoid populations or increased plasma cells were found in 18 patients (4%). A 43\/92 BMA from MDS and 7\/14 from MDS\/MPN patients had score &gt;2. Score &gt;2 had 92.5% positive predictive value for MDS\/MDS\u2010MPN diagnosis. Negative predictive value for MDS\/MDS\u2010MPN was 83% for scores under 3 and 88% for scores under 2. All but two of normal\/hospital control samples had FCM score &lt;3 (99%). Differences in scores between MDS &amp; MDS\/MPN and the control groups were statistically significant (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our one\u2010tube FCM panel can be easily applied to screening for aberrant lymphoid populations and myelodysplasia\u2010related features. MDS scores &gt;2 are highly indicative of MDS or MDS\u2010MPN. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21233","type":"journal-article","created":{"date-parts":[[2015,2,9]],"date-time":"2015-02-09T08:56:19Z","timestamp":1423472179000},"page":"253-260","source":"Crossref","is-referenced-by-count":38,"title":["Screening bone marrow samples for abnormal lymphoid populations and myelodysplasia\u2010related features with one 10\u2010color 14\u2010antibody screening tube"],"prefix":"10.1002","volume":"88","author":[{"given":"Amr","family":"Rajab","sequence":"first","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Laboratory Hematology, Laboratory Medicine Program University Health Network, Toronto General Hospital Toronto, Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Porwit","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Laboratory Hematology, Laboratory Medicine Program University Health Network, Toronto General Hospital Toronto, Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20365"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20363"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.312"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12789"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-4916"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.048421"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.097188"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2014.191"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cytob.21208"},{"key":"e_1_2_6_12_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2008"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21106"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21103"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12080"},{"key":"e_1_2_6_16_1","first-page":"419","article-title":"Ten\u2010color 14 antibody flow cytometry panel for immunophenotyping of paucicellular cytologic specimens","volume":"84","author":"Rajab A","year":"2013","journal-title":"Cytom B Clin Cytom"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21154"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21104"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404241"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.17.7089"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2010.01478.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22056"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPNCOHS92ARWRQ"},{"key":"e_1_2_6_25_1","first-page":"3023","article-title":"CD56 positive diffuse large B\u2010cell lymphoma: A case report and literature review","volume":"6","author":"Gu MJ","year":"2013","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_6_26_1","first-page":"250","article-title":"Prognostic significance of CD56 antigen expression in acute myeloid leukemia","volume":"87","author":"Di Bona E","year":"2002","journal-title":"Haematologica"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-03-492884"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2012.718341"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.81"},{"key":"e_1_2_6_30_1","unstructured":"WestersTM IrelandR KernW AlhanC BalleisenJS BettelheimP BurburyK CullenM CutlerJA Della PortaMG et al.Standardization of flow cytometry in myelodysplastic syndromes: A report from an international consortium and the European LeukemiaNet working group. 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