{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:59:30Z","timestamp":1791280770371,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,7,31]],"date-time":"2015-07-31T00:00:00Z","timestamp":1438300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Instituto de Salud Carlos III (Ministry of Economy and Competitivity, Madrid, Spain)","award":["FEDER (RD12\/0036\/0048"],"award-info":[{"award-number":["FEDER (RD12\/0036\/0048"]}]},{"name":"EuroFlow Consortium, the International Myeloma Foundation-Black Swan Research Initiative, the Red Tem\u00e1tica de Investigaci\u00f3n Cooperativa en C\u00e1ncer (RTICC)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Multiparameter flow cytometry (MFC) identification and characterization of plasma cells (PCs) is a useful tool to support diagnosis, prognostication, and monitoring of PC diseases (PCD). Currently, the number of MFC markers suited for the identification of PC remains limited. Moreover, antibody therapies against PC\u2010associated markers further compromise the utility of the most widely used reagents (e.g., CD38). Despite markers other than CD38 and CD138 are recognized as potentially useful PC\u2010identification markers, no study has comparatively evaluated their performance in combination with CD38 and CD138. Here we compared the utility of CD229, CD54, and CD319 for the identification of normal and aberrant PCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bone marrow (BM) samples from 5 healthy controls, two noninfiltrated nonHodgkin lymphoma cases and 46 PCD patients plus 3 extraosseous plasmocytomas, and normal peripheral blood (PB) specimens, were studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results showed adequate performance of all three markers once combined with CD38. In contrast, when combined with CD138 for the identification of PC, only CD229 provided a good discrimination between PCs and all other cells for all BM and PB samples analyzed; in contrast, CD54 and CD319 showed limited utility for the identification of PCs, mainly because of significant overlap of the staining for these two markers on PCs and other myeloid cells in the sample.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>From the three markers evaluated, CD229 may be considered as the most reliable marker to replace CD38 or CD138 for the identification of PCs in patients undergoing anti\u2010CD38 or anti\u2010CD138 therapy, until a better alternative is available. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21269","type":"journal-article","created":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T23:17:02Z","timestamp":1435706222000},"page":"91-100","source":"Crossref","is-referenced-by-count":54,"title":["Utility of<scp>CD<\/scp>54,<scp>CD<\/scp>229, and<scp>CD<\/scp>319 for the identification of plasma cells in patients with clonal plasma cell diseases"],"prefix":"10.1002","volume":"90","author":[{"given":"Fanny","family":"Pojero","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n del C\u00e1ncer (Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, CSIC\u2010USAL) Instituto Biosanitario de Salamanca (IBSAL), Servicio General de Citometr\u00eda y Departamento De Medicina (NUCLEUS), Universidad de Salamanca (Salamanca) Spain"},{"name":"Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi Universita\u2019 degli Studi di Palermo Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Flores\u2010Montero","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n del C\u00e1ncer (Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, CSIC\u2010USAL) Instituto Biosanitario de Salamanca (IBSAL), Servicio General de Citometr\u00eda y Departamento De Medicina (NUCLEUS), Universidad de Salamanca (Salamanca) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luzalba","family":"Sanoja","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n del C\u00e1ncer (Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, CSIC\u2010USAL) Instituto Biosanitario de Salamanca (IBSAL), Servicio General de Citometr\u00eda y Departamento De Medicina (NUCLEUS), Universidad de Salamanca (Salamanca) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Juan","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departmento de Hematolog\u00eda Hospital Universitario de Salamanca, IBSAL; IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noem\u00ed","family":"Puig","sequence":"additional","affiliation":[{"name":"Departmento de Hematolog\u00eda Hospital Universitario de Salamanca, IBSAL; IBMCC (USAL\u2010CSIC) Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Paiva","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Universidad de Navarra; Centro de Investigaciones M\u00e9dicas Aplicadas (CIMA) Pamplona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Bottcher","sequence":"additional","affiliation":[{"name":"Second Department of Medicine University Hospital of Schleswig\u2010Holstein Campus Kiel Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques J. M.","family":"van Dongen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus MC University Medical Center Rotterdam (Erasmus MC) Rotterdamthe Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n del C\u00e1ncer (Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, CSIC\u2010USAL) Instituto Biosanitario de Salamanca (IBSAL), Servicio General de Citometr\u00eda y Departamento De Medicina (NUCLEUS), Universidad de Salamanca (Salamanca) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"on behalf of the EuroFlow group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,31]]},"reference":[{"key":"e_1_2_5_2_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"McKenna AW","year":"2008"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-159624"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.15.4120"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-05-221689"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.009100"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/cyto.b.20512","article-title":"Utility of flow cytometry immunophenotyping in multiple myeloma and other clonal plasma cell\u2010related disorders","volume":"78","author":"Paiva B","year":"2010","journal-title":"Cytometry B Clin Cytom"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11080"},{"key":"e_1_2_5_9_1","first-page":"1095","article-title":"Potential role of daratumumab in the treatment of multiple myeloma","volume":"7","author":"Khagi Y","year":"2014","journal-title":"Onco Targets Ther"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-012-0362-y"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.121"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.122"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2010.036814"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2013.04.003"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.385"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2013.02.026"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08121.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-510453"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12107"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.3816\/CLM.2007.n.010"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(09)70004-0"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101302"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-09-529800"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00623.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.043125"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20547"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.018689"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21269","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21269","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T19:50:42Z","timestamp":1717962642000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,31]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1002\/cyto.b.21269"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21269","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,31]]}}}