{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T18:55:53Z","timestamp":1784400953246,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,9,8]],"date-time":"2015-09-08T00:00:00Z","timestamp":1441670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hellenic Thoracic Society"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>CD4+ cells expressing Interferon\u2010\u03b3 (IFN\u2010\u03b3), following stimulation with specific mycobacterial antigens, identified with flow cytometry (FCM\u2010CD4+IFN\u2010\u03b3+), is a new method for the diagnosis of <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (MTB) infection. The aim of this study is to investigate the performance of FCM\u2010CD4+IFN\u2010\u03b3+ in comparison with tuberculin skin test (TST) and Quantiferon TB Gold In\u2010Tube (QFT\u2010G\u2010IT) in the diagnosis of latent MTB infection (LTBI), in close contacts and in patients with rheumatic diseases under treatment with anti\u2010TNFa and other biologic agents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>TST, QFT\u2010G\u2010IT, and FCM\u2010CD4+IFN\u2010\u03b3+ were performed in 56 immunocompetent close contacts and in 65 medically immunosuppressed patients under biologic treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In close contacts, 63% were FCM\u2010CD4+IFN\u2010\u03b3+ ESAT\u20106(+), 70% FCM\u2010CD4+IFN\u2010\u03b3+ PPD(+), 41% QFT\u2010G\u2010IT(+) and 57% TST(+). FCM\u2010CD4+IFN\u2010\u03b3+ ESAT\u20106 was the only test that was strongly correlated to the exposure time to infection. In the immunosuppressed group, 49% were FCM\u2010CD4+IFN\u2010\u03b3+ ESAT\u20106(+), 62% FCM\u2010CD4+IFN\u2010\u03b3+ PPD(+), 4.6% QFT\u2010G\u2010IT(+), and 18% TST(+).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>FCM\u2010CD4+IFN\u2010\u03b3+ assays are more sensitive than QFT\u2010G\u2010IT and TST for the diagnosis of LTBI in close contacts and in immunosuppressed patients under anti\u2010TNF\u2010a treatment. FCM\u2010CD4+IFN\u2010\u03b3+ is not affected by the chronic use of biologic agents. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21275","type":"journal-article","created":{"date-parts":[[2015,7,22]],"date-time":"2015-07-22T22:43:28Z","timestamp":1437605008000},"page":"303-311","source":"Crossref","is-referenced-by-count":10,"title":["Flow cytometry analysis of <scp>CD4+IFN\u2010\u03b3+ T\u2010<\/scp>cells for the diagnosis of mycobacterium tuberculosis infection"],"prefix":"10.1002","volume":"90","author":[{"given":"Chrysovalantis V.","family":"Papageorgiou","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Locus Medicus S.A. 246 Mesogeion Ave Athens 15561 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Anastasopoulos","sequence":"additional","affiliation":[{"name":"1st Department of Pulmonary Medicine Sotiria Hospital of Chest Diseases 152 Mesogeion Ave Athens 11527 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Ploussi","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology and Immunology Locus Medicus S.A. 246 Mesogeion Ave Athens 15561 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michail","family":"Leventopoulos","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology and Immunology Locus Medicus S.A. 246 Mesogeion Ave Athens 15561 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Karabela","sequence":"additional","affiliation":[{"name":"Microbiology Department, National Reference Laboratory for Mycobacteria Sotiria Hospital of Chest Diseases 152 Mesogeion Ave Athens 11527 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyriakos","family":"Fotiadis","sequence":"additional","affiliation":[{"name":"Rheumatology Department General Hospital of Athens \u2018G. Gennimatas\u2019 154 Mesogeion Ave Athens 11527 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apostolos","family":"Papavasileiou","sequence":"additional","affiliation":[{"name":"Clinic for Multidrug\u2010Resistant Tuberculosis Sotiria Hospital of Chest Diseases 152 Mesogeion Ave Athens 11527 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelos","family":"Vogiatzakis","sequence":"additional","affiliation":[{"name":"Microbiology Department, National Reference Laboratory for Mycobacteria Sotiria Hospital of Chest Diseases 152 Mesogeion Ave Athens 11527 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Ioakeimidis","sequence":"additional","affiliation":[{"name":"Rheumatology Department General Hospital of Athens \u2018G. Gennimatas\u2019 154 Mesogeion Ave Athens 11527 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelos D.","family":"Gritzapis","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology and Immunology Locus Medicus S.A. 246 Mesogeion Ave Athens 15561 Greece"},{"name":"Department of Virology Saint Savas Cancer Hospital 171 Alexandras Ave Athens 11522 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos","family":"Poulakis","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Locus Medicus S.A. 246 Mesogeion Ave Athens 15561 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,8]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"355","article-title":"Intradermo\u2010reaction de la tuberculine","volume":"147","author":"Mantoux C.","year":"1908","journal-title":"Comptes Rendus De L'academie Des Sci"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.10-0366"},{"key":"e_1_2_6_4_1","first-page":"1","article-title":"IGRA Expert Committee; Centers for Disease Control and Prevention (CDC). Updated guidelines for using interferon gamma release assays to detect Mycobacterium tuberculosis infection\u2014United States, 2010","volume":"59","author":"Mazurek GH","year":"2010","journal-title":"MMWR Recomm Rep"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20614"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20503"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20007"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2012.08.020"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20381"},{"key":"e_1_2_6_10_1","first-page":"1","article-title":"Guidelines for the investigation of contacts of persons with infectious tuberculosis Recommendations from the National Tuberculosis Controllers Association and CDC","volume":"54","author":"National Tuberculosis Controllers Association; Centers for Disease Control and Prevention (CDC)","year":"2005","journal-title":"MMWR Recomm Rep"},{"key":"e_1_2_6_11_1","volume-title":"Centre for Clinical Practice at NICE (UK) Tuberculosis: clinical diagnosis and management of tuberculosis, and measures for its prevention and control","author":"National Collaborating Centre for Chronic Conditions (UK)","year":"2011"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.11-3157"},{"key":"e_1_2_6_13_1","unstructured":"Cellestis. QuantiFERON\u2010TB Gold In\u2010Tube package insert. Available at:http:\/\/www.cellestis.com.2009."},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfl416"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20602"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kem351"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8479(95)90554-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)05115-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70227-5"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803579"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.022649799"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1468"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2235593100"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.6.3570"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000735"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21275","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21275","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T20:59:06Z","timestamp":1696539546000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,8]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/cyto.b.21275"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21275","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,8]]}}}