{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:50:22Z","timestamp":1777510222271,"version":"3.51.4"},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T00:00:00Z","timestamp":1444089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p>CD180, a related member of the Toll\u2010like receptor family, is lost or underexpressed at the plasma membrane in circulating cells of various B\u2010cell lymphomas except marginal zone lymphomas (MZL). In order to confirm its clinical relevance in routine analysis, we evaluated prospectively the expression of CD180 in 236 patients from 5 French University Hospital laboratories on behalf of the GEIL. Highly comparable results were obtained in all centers using the EuroFlow standardization protocol. We observed that CD180 median fluorescence (MdFI) was significantly higher in MZL and hairy cell leukaemia (HCL) compared to all other B\u2010cell proliferations (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). CD180 intensity could distinguish lymphomas with numerous villous lymphocytes from other MZL. ROC curve analysis identified a CD180 MdFI threshold for which the diagnosis of MZL could be assessed with 77% sensitivity and 92% specificity. This study showed that CD180 can be considered as a single positive robust marker of MZL and should be therefore included in flow cytometry panels for the diagnosis of mature B\u2010cell neoplasms. Harmonization process is of great interest in order to evaluate new markers in multicentric studies and to set up decisional thresholds. \u00a9 2015 International Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.21325","type":"journal-article","created":{"date-parts":[[2015,9,19]],"date-time":"2015-09-19T02:05:38Z","timestamp":1442628338000},"page":"462-466","source":"Crossref","is-referenced-by-count":21,"title":["<scp>CD<\/scp>180 expression in <scp>B<\/scp>\u2010cell lymphomas: A multicenter <scp>GEIL<\/scp> study"],"prefix":"10.1002","volume":"90","author":[{"given":"Caroline","family":"Mayeur\u2010Rousse","sequence":"first","affiliation":[{"name":"Laboratoire D'h\u00e9matologie CHU De Hautepierre Strasbourg France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Guy","sequence":"additional","affiliation":[{"name":"Laboratoire D'h\u00e9matologie CHU De Dijon Dijon 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