{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:39:16Z","timestamp":1765294756293},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,10,10]],"date-time":"2015-10-10T00:00:00Z","timestamp":1444435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The ARUP Institute for Experimental Pathology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The eosin\u20105\u2032maleimide (EMA) binding test has been studied extensively for the detection of hereditary spherocytosis (HS). Its performance characteristics have been compared to NaCl\u2010based or glycerol lysis\u2010based red cell osmotic fragility tests and cryohemolysis. HS samples are also better identified when both mean channel fluorescence (MCF) of EMA relative to controls and the coefficient of variation (CV) are analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We looked at 65 normal controls including 30 adults 25\u201065 years old and 35 newborns and 12 HS cases. In addition to the MCF and the CV, we used a side scatter (SSC) vs. EMA fluorescence gate or \u201cfootprint\u201d to depict where normal erythrocytes should appear. Erythrocytes that have reduced band 3 protein appear outside of the footprint.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In our study, newborn data did not cluster with the samples from working age individuals. The MCF and the CVs of normal newborns were higher than normal adult group. However, the footprint data of normal samples relative to their controls was around 99.5% for each group, because the footprint was moved to fit the pattern of the normal.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The inclusion of footprint parameter will help in better standardization as well as implementation of this test across different age groups as well as different instruments. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21326","type":"journal-article","created":{"date-parts":[[2015,9,19]],"date-time":"2015-09-19T02:05:33Z","timestamp":1442628333000},"page":"512-516","source":"Crossref","is-referenced-by-count":8,"title":["Improved harmonization of eosin\u20105\u2010maleimide binding test across different instruments and age groups"],"prefix":"10.1002","volume":"90","author":[{"given":"Archana M.","family":"Agarwal","sequence":"first","affiliation":[{"name":"Department of Pathology University of Utah Salt Lake City, Utah 84112"},{"name":"Associated Regional and University Pathologists (ARUP) Laboratories Salt Lake City Utah 84108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Liew","sequence":"additional","affiliation":[{"name":"Associated Regional and University Pathologists (ARUP) Laboratories Salt Lake City Utah 84108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto H.","family":"Nussenzveig","sequence":"additional","affiliation":[{"name":"Associated Regional and University Pathologists (ARUP) Laboratories Salt Lake City Utah 84108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikhil","family":"Sangle","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Dental Sciences Building Western University London, Ontario 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