{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T13:52:21Z","timestamp":1779285141290,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T00:00:00Z","timestamp":1450051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"\u201cFrankfurter Stiftung f\u00fcr krebskranke Kinder\u201d and \u201cHilfe f\u00fcr krebskranke Kinder Frankfurt e.V.\u201d, as well as the \u201cLOEWE Center for Cell and Gene Therapy Frankfurt\u201d"},{"DOI":"10.13039\/501100003495","name":"Hessisches Ministerium f\u00fcr Wissenschaft und Kunst (HMWK)","doi-asserted-by":"crossref","award":["III L 5-518\/17.004 (2013)"],"award-info":[{"award-number":["III L 5-518\/17.004 (2013)"]}],"id":[{"id":"10.13039\/501100003495","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The depletion of TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> T cells and CD19<jats:sup>+<\/jats:sup> B cells is a graft purification method for haploidentical stem cell transplantation (HSCT) retaining stem cells, NK cells and TCR\u03b3\u03b4<jats:sup>+<\/jats:sup> T cells. To avoid treatment\u2010related occurrence of severe GvHD a precise quantification of residual TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> T cells in the graft is of essential importance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nine stem cell grafts were purified immunomagnetically on a CliniMACS device and flow cytometric quality control (QC) was performed before and after TCR\u03b1\u03b2\/CD19\u2010depletion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As a challenge a new 10\u2010color QC\u2010panel was established, which enables accurate quantification of the graft composition. The binding sites of residual TCR\u03b1\u03b2<jats:sup>+<\/jats:sup> T and CD19<jats:sup>+<\/jats:sup> B cells were at least partly occupied by depletion antibodies impeding flow cytometric analysis. Based on respective controls and an assumed variation coefficient of 18%, the detection limit of residual TCR\u03b1\u03b2 T cells was 1 cell\/\u00b5l. and 0.002% of CD45<jats:sup>+<\/jats:sup> cells. Log\u2010depletion of TCR\u03b1\u03b2 and CD19 cells was \u22123.9 and \u22123.3, respectively. The recovery was 82.1%, 67.1% and 72.7% for stem cells, NK cells and for TCR\u03b3\u03b4<jats:sup>+<\/jats:sup> T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In clinical use this method may help to improve transplantation outcome, due to the correct application of the desired stem cell and the limited T cell dose. The panel is designed for the QC following TCR\u03b1\u03b2\/CD19\u2010depletion but is adaptable to other depletion strategies as well. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21328","type":"journal-article","created":{"date-parts":[[2015,9,29]],"date-time":"2015-09-29T00:04:43Z","timestamp":1443485083000},"page":"126-135","source":"Crossref","is-referenced-by-count":11,"title":["Generation and flow cytometric quality control of clinical\u2010scale <scp>TCR\u03b1\u03b2\/CD<\/scp>19\u2010depleted grafts"],"prefix":"10.1002","volume":"92","author":[{"given":"Melanie","family":"Bremm","sequence":"first","affiliation":[{"name":"Clinic for Pediatric and Adolescent Medicine University Hospital Frankfurt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Cappel","sequence":"additional","affiliation":[{"name":"Clinic for Pediatric and Adolescent Medicine University Hospital Frankfurt 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