{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T15:15:25Z","timestamp":1725290125516},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T00:00:00Z","timestamp":1450051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>We present a pre B\u2010ALL patient with the rare clinical manifestation of extramedullary disease, and a normal hemogram. This patient's blasts expressed bright CD45 and high side scatter (SSc) placing the cells in the monocyte gate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples from peripheral blood and bone marrow (BM) aspirate from a 50\u2010year\u2010old female patient were immunophenotyped by multiparametric flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Flow cytometry studies of the BM aspirate showed a large monocyte gate with 90\u201095% of the cells expressing an abnormal B cell phenotype. Peripheral white blood cells count was normal and cytogenetic analysis of the BM revealed a normal karyotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It was not possible, based on CD45\/SSc to identify a lymphoblast population in this pre B\u2010ALL patient. Although bright expression of CD45 B\u2010ALL blasts has been associated with poor prognosis to the best of our knowledge, the combination of bright CD45 blasts with high SSc has not been reported. As CD45 expression vs. SSc is routinely measured in the diagnostics of acute leukemias, a possible association between CD45 bright positivity and extramedullary disease or prognosis warrants further exploration. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21329","type":"journal-article","created":{"date-parts":[[2015,9,29]],"date-time":"2015-09-29T00:02:37Z","timestamp":1443484957000},"page":"161-164","source":"Crossref","is-referenced-by-count":3,"title":["Adult pre B\u2010cell acute lymphoblastic leukemia with unusually large proportion of bone marrow <scp>CD<\/scp>45 bright\/high <scp>SS<\/scp>c blasts"],"prefix":"10.1002","volume":"92","author":[{"given":"Yael","family":"Shahal\u2010Zimra","sequence":"first","affiliation":[{"name":"Hematology Laboratory, Flow Cytometry Division Hematology Institute; Tel Aviv University. Beilinson Hospital, Rabin Medical Center Petah Tikva Israel"},{"name":"Felsenstein Medical Research Center, Sackler School of Medicine Tel Aviv University. Beilinson Hospital, Rabin Medical Center Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohar","family":"Rotem","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, Flow Cytometry Division Hematology Institute; Tel Aviv University. Beilinson Hospital, Rabin Medical Center Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Chezar","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, Flow Cytometry Division Hematology Institute; Tel Aviv University. Beilinson Hospital, Rabin Medical Center Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nino","family":"Oniashvili","sequence":"additional","affiliation":[{"name":"Cytogenetic Institute; Tel Aviv University, Beilinson Hospital, Rabin Medical Center Petah Tikva Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avi","family":"Leader","sequence":"additional","affiliation":[{"name":"Institute of Hematology, Davidoff Cancer Center, Rabin Medical Center Petah Tikva and Sackler School of Medicine Tel\u2010Aviv University Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pia","family":"Raanani","sequence":"additional","affiliation":[{"name":"Institute of Hematology, Davidoff Cancer Center, Rabin Medical Center Petah Tikva and Sackler School of Medicine Tel\u2010Aviv University Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Rabizadeh","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, Flow Cytometry Division Hematology Institute; Tel Aviv University. 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