{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T06:38:17Z","timestamp":1768804697835,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,12,11]],"date-time":"2015-12-11T00:00:00Z","timestamp":1449792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Altered Toll\u2010like receptor (TLR) expression levels and\/or mutations in its signaling pathway (such as MyD88 mutation) contribute to the pathogenesis of lymphoproliferative disorders (LPD). CD180 is an orphan member of the TLR family that modulates the signaling of several TLRs, but only limited studies have evaluated its expression by flow cytometry (FCM) in LPD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using a multiparameter FCM approach, we have assessed CD180 mean fluorescence intensity (MFI) in lymph nodes (LNs) and peripheral blood (PB) samples obtained from patients with follicular lymphoma (FL; LN\/PB, <jats:italic>n<\/jats:italic>\u2009=\u200944\/<jats:italic>n<\/jats:italic>\u2009=\u200915), chronic lymphocytic leukemia (CLL, <jats:italic>n<\/jats:italic>\u2009=\u200926\/<jats:italic>n<\/jats:italic>\u2009=\u200921), mantle cell lymphoma (MCL, <jats:italic>n<\/jats:italic>\u2009=\u200913\/<jats:italic>n<\/jats:italic>\u2009=\u200917), and marginal zone lymphoma (MZL, <jats:italic>n<\/jats:italic>\u2009=\u200916\/<jats:italic>n<\/jats:italic>\u2009=\u200912). Specimens from non\u2010tumoral PB and LN (<jats:italic>n<\/jats:italic>\u2009=\u20098\/<jats:italic>n<\/jats:italic>\u2009=\u200912) were used as controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the LN specimens, FL and control B\u2010cells showed similar CD180 expression (MFI\u2009=\u20091,049 vs. 1,381, <jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05; Mann\u2013Whitney <jats:italic>U<\/jats:italic>\u2010test). This level was markedly lower in the other LPDs, MCL (MFI\u2009=\u2009396, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), or CLL (MFI\u2009=\u2009502 <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), and similar to MZL (MFI\u2009=\u2009858, <jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). However, the CD180 expression of FL B\u2010cells assessed in PB was dim and\/or negative, in the same range as MCL and CLL (FL MFI\u2009=\u2009453, MCL MFI\u2009=\u2009305, CLL MFI\u2009=\u2009420, <jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05) but lower than in MZL (MFI\u2009=\u2009895, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Therefore, these results suggest a modulation of CD180 expression by neoplastic FL B\u2010cells based on the anatomical compartment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These FCM data confirm the usefulness of CD180 in the accurate diagnosis of LPDs and emphasize the need to interpret this marker according to the origin of the sample. \u00a9 2015 Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21331","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T09:07:41Z","timestamp":1445332061000},"page":"433-439","source":"Crossref","is-referenced-by-count":12,"title":["<scp>CD<\/scp>180 overexpression in follicular lymphoma is restricted to the lymph node compartment"],"prefix":"10.1002","volume":"90","author":[{"given":"Fan\u00e9lie","family":"Mestrallet","sequence":"first","affiliation":[{"name":"Laboratoire D'h\u00e9matologie Cellulaire Pierre\u2010B\u00e9nite France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Sujobert","sequence":"additional","affiliation":[{"name":"Laboratoire D'h\u00e9matologie Cellulaire Pierre\u2010B\u00e9nite France"},{"name":"UMR5239 Pathologies Des Cellules Lympho\u00efdes, Universit\u00e9 Claude Bernard Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e9mentine","family":"Sarkozy","sequence":"additional","affiliation":[{"name":"UMR5239 Pathologies Des Cellules Lympho\u00efdes, Universit\u00e9 Claude Bernard Lyon France"},{"name":"Service D'h\u00e9matologie Centre Hospitalier Lyon\u2010Sud\/Hospices Civils De Lyon Pierre\u2010B\u00e9nite France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Traverse\u2010Glehen","sequence":"additional","affiliation":[{"name":"UMR5239 Pathologies Des Cellules Lympho\u00efdes, Universit\u00e9 Claude Bernard Lyon France"},{"name":"Service d'Anatomie\u2010Pathologique Pierre\u2010B\u00e9nite France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyne","family":"Callet\u2010Bauchu","sequence":"additional","affiliation":[{"name":"Laboratoire D'h\u00e9matologie Cellulaire Pierre\u2010B\u00e9nite France"},{"name":"UMR5239 Pathologies Des Cellules Lympho\u00efdes, Universit\u00e9 Claude Bernard Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Pierre","family":"Magaud","sequence":"additional","affiliation":[{"name":"Laboratoire D'h\u00e9matologie Cellulaire Pierre\u2010B\u00e9nite France"},{"name":"UMR5239 Pathologies Des Cellules Lympho\u00efdes, Universit\u00e9 Claude Bernard Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Salles","sequence":"additional","affiliation":[{"name":"UMR5239 Pathologies Des Cellules Lympho\u00efdes, Universit\u00e9 Claude Bernard Lyon France"},{"name":"Service D'h\u00e9matologie Centre Hospitalier Lyon\u2010Sud\/Hospices Civils De Lyon Pierre\u2010B\u00e9nite France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucile","family":"Baseggio","sequence":"additional","affiliation":[{"name":"Laboratoire D'h\u00e9matologie Cellulaire Pierre\u2010B\u00e9nite France"},{"name":"UMR5239 Pathologies Des Cellules Lympho\u00efdes, Universit\u00e9 Claude Bernard Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2013.04.002"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.4049\/jimmunol.140.12.4071","article-title":"Antibody to a novel 95\u2010kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation","volume":"140","author":"Valentine MA","year":"1988","journal-title":"J 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