{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T03:35:41Z","timestamp":1768793741092,"version":"3.49.0"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T00:00:00Z","timestamp":1454630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>B\u2010lymphoblastic leukemias (B\u2010LBL) with combined IGH\/BCL2 and MYC rearrangement are rare and their clinical, cytogenetic and immunophenotypic features are not well characterized. Here, we describe a case of a 61\u2010year\u2010old woman with B\u2010LBL associated with these cytogenetic alterations and present a review of the literature of this disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four\u2010color flow cytometry (FC) was performed on a BD FACSCanto II flow cytometer. Data were analyzed with BD FACSDiva software. Cytogenetic, fluorescence in situ hybridization (FISH), and molecular studies were performed by conventional methods. A review of the literature was performed by a PubMed\u2010assisted search.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Including our case, eight B\u2010LBLs associated with a documented \u201cdouble\u2010hit\u201d karyotype (IGH\/BCL2 and 8q24\/MYC rearrangement) were identified in the literature (male\/female 2\/6, age 15\u201065). Three occurred de\u2010novo, and five had a history of a CD10+ B\u2010cell lymphoma. The typical immunophenotype was CD10, CD19, TdT positive, and negative for CD34 and surface immunoglobulin (Ig), established either by FC or immunohistochemistry. Seven cases were CD20\u2010, and one case was CD20+. Translocation partners of MYC varied, and included IGH, lambda light chain, and an unknown gene on chromosome 9. Prognosis was poor with median survival of five months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Patients with B\u2010LBL associated with a combined IGH\/BCL2 and MYC rearrangement often have a history of a mature B\u2010cell lymphoma. The immunophenotype of these cases is different from that of mature \u201cdouble\u2010hit\u201d lymphomas; FC is essential to differentiate the B\u2010LBL cases from the leukemic phase of mature B\u2010cell lymphomas. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21334","type":"journal-article","created":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T23:46:23Z","timestamp":1446248783000},"page":"310-314","source":"Crossref","is-referenced-by-count":12,"title":["Immunophenotypic and cytogenetic findings of <scp>B<\/scp>\u2010lymphoblastic leukemia\/lymphoma associated with combined <scp>IGH\/BCL<\/scp>2 and <scp>MYC<\/scp> rearrangement"],"prefix":"10.1002","volume":"92","author":[{"given":"Katalin","family":"Kelemen","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Hospital Phoenix Arizona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaclyn","family":"Holden","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Hospital Phoenix Arizona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Hospital Phoenix Arizona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Davion","sequence":"additional","affiliation":[{"name":"Department of Pathology the Billings Clinic Billings Montana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan S.","family":"Robetorye","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Hospital Phoenix Arizona"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,5]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_3_3_1","first-page":"265","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Kluin PM","year":"2008"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP7YLDTJPLCE5F"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPL11MAHISIJBQ"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198805263182106"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.22.8548"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/NKK3FEX2BE5L7EKB"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.3960\/jslrt.52.113"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.22.7166"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870020211"},{"key":"e_1_2_3_12_1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1182\/blood.V82.1.231.bloodjournal821231","article-title":"CMYC translocations in de novo B\u2010cell lineage acute leukemias with t(14;18)(cell lines Karpas 231 and 353)","volume":"82","author":"Nacheva E","year":"1993","journal-title":"Blood"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.25607"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.21.1318"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21101"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21334","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21334","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T22:23:31Z","timestamp":1696544611000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,5]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1002\/cyto.b.21334"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21334","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,5]]}}}