{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:38:06Z","timestamp":1768459086625,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T00:00:00Z","timestamp":1459209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Flow cytometry is an important tool for identification of neoplastic T\u2010cells, but immunophenotypic abnormalities are often subtle and must be distinguished from nonneoplastic subsets. Use of internal control (IC) T\u2010cells in the evaluation for T\u2010cell neoplasms was explored, both as a quality measure and as a reference for evaluating abnormal antigen expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>All peripheral blood specimens (3\u2010month period), or those containing abnormal T\u2010cells (29\u2010month period), stained with CD45 V500, CD2 V450, CD3 PE\u2010Cy7, CD7 PE, CD4 Per\u2010CP\u2010Cy5.5, CD8 APC\u2010H7, CD56 APC, CD16&amp;57 FITC, were evaluated. IC T\u2010cells were identified (DIVA, BD Biosciences) and median fluorescence intensity (MFI) recorded. Selected files were merged and reference templates generated (Infinicyt, Cytognos).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IC T\u2010cells were present in all specimens, including those with abnormal T\u2010cells, but subsets were less well\u2010represented. IC T\u2010cell CD3 MFI differed between instruments (<jats:italic>p<\/jats:italic>\u2009=\u20090.0007) and subsets (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), but not specimen categories, and served as a longitudinal process control. Merged files highlighted small unusual IC\u2010T subsets: CD2+(dim) (0.25% total), CD2\u2212 (0.03% total). An IC reference template highlighted neoplastic T\u2010cells, but was limited by staining variability (IC CD3 MFI reference samples different from test (<jats:italic>p<\/jats:italic>\u2009=\u20090.003)).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>IC T\u2010cells present in the majority of specimens can serve as positive and longitudinal process controls. Use of IC T\u2010cells as an internal reference is limited by variable representation of subsets. Analysis of merged IC T\u2010cells from previously analyzed patient samples can alert the interpreter to less\u2010well\u2010recognized non\u2010neoplastic subsets. However, application of a merged file IC reference template was limited by staining variability. \u00a9 2016 Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21335","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T08:46:41Z","timestamp":1446540401000},"page":"404-414","source":"Crossref","is-referenced-by-count":8,"title":["Use of internal control<scp>T<\/scp>\u2010cell populations in the flow cytometric evaluation for<scp>T<\/scp>\u2010cell neoplasms"],"prefix":"10.1002","volume":"90","author":[{"given":"Alicia M.","family":"Hunt","sequence":"first","affiliation":[{"name":"Department of Pathology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy","family":"Shallenberger","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Medical Center Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P.","family":"Ten Eyck","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiona E.","family":"Craig","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Pittsburgh School of Medicine Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,29]]},"reference":[{"key":"e_1_2_5_2_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2008"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21107"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21106"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19971015)30:5<214::AID-CYTO2>3.0.CO;2-H"},{"key":"e_1_2_5_7_1","unstructured":"KeeneyM OldakerT.CLIA Compliance Manual second edition. 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