{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:43:08Z","timestamp":1762101788546},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T00:00:00Z","timestamp":1450742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RC1\u2010HL0995828","P30\u2010CA016520"],"award-info":[{"award-number":["RC1\u2010HL0995828","P30\u2010CA016520"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K12 HL083772\u201001"],"award-info":[{"award-number":["K12 HL083772\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>We previously reported the development of a novel high dimensional cytomic assay, the Vascular Health Profile (VHP) based on measurements of angiogenic circulating hematopoietic stem and progenitor cells (CHSPC<jats:sup>Ang<\/jats:sup>) and extracellular vesicles (EVs), that discovered a unique signature, differentiating the vascular status of diabetics and normal healthy controls. Here, we present data from a 3\u2010year follow\u2010up to evaluate the power of the VHP to identify individuals at risk for cardiovascular (CV) events.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The original data were generated as previously described by measuring a broad panel of progenitor cells and EVs and profiled using cytometric fingerprinting. Subjects were classified into groups according to the occurrence of adjudicated CV events including myocardial infarction, stroke, major adverse cardiovascular events, revascularization, and irregular rhythm. Cross\u2010validated Linear Discriminate Analysis (LDA) models were constructed and used to predict the occurrence of events, and were evaluated for predictive accuracy (AUC, area under the curve) using receiver operating characteristic (ROC) analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Over the period of this analysis, follow\u2010up data was obtained on 87 subjects, with 32 events occurring overall, and only in the diabetic group. In all cases, the VHP added significant predictive power, in the form of ROC analysis, for all evaluated outcomes with the exception of irregular rhythm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The VHP, a relatively simple blood test, can provide sensitive and clinically relevant information on the vascular status of a patient that may be useful for a variety of applications including drug development, clinical risk assessment, and companion diagnostics. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21337","type":"journal-article","created":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T15:58:52Z","timestamp":1447430332000},"page":"258-265","source":"Crossref","is-referenced-by-count":3,"title":["<scp>V<\/scp>ascular <scp>H<\/scp>ealth <scp>P<\/scp>rofile predicts cardiovascular outcomes in patients with diabetes"],"prefix":"10.1002","volume":"92","author":[{"given":"Wade T.","family":"Rogers","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Perelman School of Medicine at the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lifeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Section of Vascular Medicine Division of Cardiovascular Disease Department of Medicine Perelman School of Medicine at the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Welden","sequence":"additional","affiliation":[{"name":"Section of Vascular Medicine Division of Cardiovascular Disease Department of Medicine Perelman School of Medicine at the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Krieger","sequence":"additional","affiliation":[{"name":"Section of Vascular Medicine Division of Cardiovascular Disease Department of Medicine Perelman School of Medicine at the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Rickels","sequence":"additional","affiliation":[{"name":"Division of Endocrinology Diabetes and Metabolism Department of Medicine Perelman School of Medicine at the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonni S.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Perelman School of Medicine at the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Emile R.","family":"Mohler","sequence":"additional","affiliation":[{"name":"Section of Vascular Medicine Division of Cardiovascular Disease Department of Medicine Perelman School of Medicine at the University of Pennsylvania Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e31820a55f5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(93)90050-B"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2004.11.067"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X13499773"},{"key":"e_1_2_6_6_1","article-title":"Identification of endothelial cells and progenitor cell subsets in human peripheral blood","author":"Estes ML","journal-title":"Curr Protoc Cytom"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21095"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20545"},{"key":"e_1_2_6_9_1","first-page":"11","article-title":"A bioconductor package for fingerprinting flow cytometric data","volume":"193947","author":"Rogers WT","year":"2009","journal-title":"Adv bioinform"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.19.2570"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22109"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20011101)45:3<194::AID-CYTO1163>3.0.CO;2-C"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20040"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20528"},{"key":"e_1_2_6_15_1","volume-title":"FlowFP: Fingerprinting for Flow Cytometry. R package version 1.8.0","author":"Holyst H","year":"2011"},{"key":"e_1_2_6_16_1","unstructured":"B Ellis P Haaland F Hahne N Le Meur N Gopalakrishnan J Spidlen 2011"},{"key":"e_1_2_6_17_1","unstructured":"WandM.KernSmooth: Functions for kernel smoothing for Wand & Jones. R package version 2.23\u20106.2011. web link:http:\/\/cran.us.r-project.org\/src\/contrib\/Archive\/KernSmooth\/KernSmooth_2.23-6.tar.gz."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2007.09.011"},{"key":"e_1_2_6_19_1","volume-title":"R: A language and environment for statistical computing","year":"2011"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043814"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.05.022"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(85)80518-0"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2009.476"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.07.013"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20669"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.02.057"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ehj.2004.03.026"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.226456"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.109.200998"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20551"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000249639.36915.04"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1262-3636(08)70100-9"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8703(03)00103-0"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehq478"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21681"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1159\/000045292"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2005.01707.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.02.047"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0710-9"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2009.06.037"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022287"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.02.065"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000124065.31211.6E"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21337","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21337","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T22:23:10Z","timestamp":1696544590000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,22]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1002\/cyto.b.21337"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21337","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,22]]}}}