{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:11:22Z","timestamp":1791353482305,"version":"4.3.1"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T00:00:00Z","timestamp":1452556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["13\u201022777S)"],"award-info":[{"award-number":["13\u201022777S)"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cytomegalovirus (CMV) specific T\u2010cells are known to provide long\u2010term control of CMV reactivation, which is a frequent complication of hematopoietic stem cell transplantation. We have studied 58 pediatric patients after hematopoietic stem cell transplantation who suffered from CMV reactivation to reveal which functional T cell subset is best correlating with successful reactivation resolution and which protects from reactivation episode.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Detection of 30 combinatorial subsets of four types of response to ex vivo CMV stimulation (IFN\u03b3 secretion, IL\u20102 secretion, CD40L upregulation and degranulation) that were detectable on either CD8+ or CD4+ T cells through flow cytometry intracellular cytokine staining was used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that the presence of CD8+ dual positive (IFN\u03b3+ and IL\u20102+) cells is the most accurate functional parameter that can predict fast resolution of CMV reactivation. Next, we show that the presence of CD8+ dual positive (IFN\u03b3+ and IL\u20102+) and CD8+ IFN\u03b3+ cells provides a protective effect (a hazard risk of 0.28 (confidence interval 0.18 \u2013 0.43) and 0.45 (CI 0.27 \u2013 0.75), respectively) and the presence of corticotherapy increases the risk of reactivation (HR 2.47 (CI 1.82\u20103.36)). Thus, a patient without corticotherapy and with both of the critical T cell subsets present has a cumulative 19.6 times lower risk of developing CMV reactivation than a patient on corticotherapy and without CD8+ dual positive (IFN\u03b3+ and IL\u20102+) or CD8+ IFN\u03b3+ cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We have established parameters of CMV specific functional response ex vivo that can be used in assisting clinical management of patients with CMV reactivation. \u00a9 2015 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21348","type":"journal-article","created":{"date-parts":[[2015,12,9]],"date-time":"2015-12-09T07:08:27Z","timestamp":1449644907000},"page":"380-388","source":"Crossref","is-referenced-by-count":21,"title":["Appearance of cytomegalovirus\u2010specific <scp>T<\/scp>\u2010cells predicts fast resolution of viremia post hematopoietic stem cell transplantation"],"prefix":"10.1002","volume":"92","author":[{"given":"Ond\u0159ej","family":"Pel\u00e1k","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol V Uvalu 84, Prague 150 06 Czech Republic"},{"name":"CLIP\u2013Childhood Leukemia Investigation Prague, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol V Uvalu 84, Prague 150 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Stuchl\u00fd","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol V Uvalu 84, Prague 150 06 Czech Republic"},{"name":"CLIP\u2013Childhood Leukemia Investigation Prague, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol V Uvalu 84, Prague 150 06 Czech 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Hematology and Oncology, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol V Uvalu 84, Prague 150 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Sedl\u00e1\u010dek","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol V Uvalu 84, Prague 150 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Form\u00e1nkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine Charles University Prague and University Hospital Motol V Uvalu 84, Prague 150 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Star\u00fd","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine 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