{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T11:59:22Z","timestamp":1753271962500},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,5,6]],"date-time":"2016-05-06T00:00:00Z","timestamp":1462492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Bone marrow analysis by flow cytometry is part of the routine diagnosis of hematological disorders in medical laboratories. Differential leukocyte count and identification of abnormal cell subsets is currently performed through morphological examination on bone marrow smears by skilled cytologists. In this work, we propose a single 8\u2010color tube for providing equivalent information, using flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>99 bone marrow samples were classified into 2 groups, (i) 51 samples, obtained from either healthy donors (<jats:italic>n<\/jats:italic>\u2009=\u20094) or patients with various diseases at diagnosis or during remission that did not present a hematological malignancy (<jats:italic>n<\/jats:italic>\u2009=\u200947), and (ii) 48 pathological samples with quantitative and\/or qualitative abnormalities. A panel of eight antibodies\u2014CD3\u2010FITC\/CD10\u2010PE\/CD38\u2010PerCP\u2010Cy5.5\/CD19\u2010PECy7\/CD36\u2010APC\/CD16\u2010APC\u2010H7\/CD34\u2010BV421\/CD45\u2010V500\u2014was tested to identify the main cell subsets at different stages of maturation using a FACSCanto\u2010II analyzer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We first proposed a strategy of sequential gating leading to the identification of 14 leukocyte subsets, that is, erythroblasts, monocytes, B\u2010lymphoid cells from hematogones to plasma\u2010cells (5 subsets), T\u2010 and NK\u2010cells, polymorphonuclear cells (neutrophils, eosinophils, and basophils), myeloblasts and other immature granular cells. This approach was validated by comparing flow cytometry and microscopic morphological examination, both in cases of normal and abnormal samples. Interestingly, cell identification, and numeration by flow cytometry was easy to perform and highly reproducible.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A very simple, rapid, and reproducible flow cytometric approach, using a combination of eight antibodies allows determination of the cellular composition of bone marrow with high precision. \u00a9 2016 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21369","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T00:47:22Z","timestamp":1458348442000},"page":"299-309","source":"Crossref","is-referenced-by-count":8,"title":["One tube with eight antibodies for 14\u2010part bone marrow leukocyte differential using flow cytometry"],"prefix":"10.1002","volume":"92","author":[{"given":"Marie\u2010Christine","family":"Jacob","sequence":"first","affiliation":[{"name":"Universit\u00e9 Grenoble\u2010Alpes Grenoble 38000 France"},{"name":"CNRS UMR 5309 and INSERM, U1209, Institut Albert Bonniot Grenoble 38706 France"},{"name":"Department of Immunology CHU Grenoble La Tronche F\u201038700 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Souvignet","sequence":"additional","affiliation":[{"name":"Department of Immunology CHU Grenoble La Tronche F\u201038700 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Pont","sequence":"additional","affiliation":[{"name":"Department of Hematology CHU Grenoble La Tronche F\u201038700 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7oise","family":"Solly","sequence":"additional","affiliation":[{"name":"Department of Hematology CHU Saint Etienne Saint Priest en Jarez F\u201042270 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Mondet","sequence":"additional","affiliation":[{"name":"Department of Hematology CHU Grenoble La Tronche F\u201038700 France"},{"name":"Therex, TIMC\u2010IMAG, CNRS Universit\u00e9 Joseph Fourier La Tronche F\u201038700 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanae","family":"Kesr","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Diderot\u2010CHU Saint Etienne Saint Priest en Jarez F\u201042270 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Pernollet","sequence":"additional","affiliation":[{"name":"Department of Immunology CHU Grenoble La Tronche F\u201038700 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"Dumestre\u2010Perard","sequence":"additional","affiliation":[{"name":"Department of Immunology CHU Grenoble La Tronche F\u201038700 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Campos","sequence":"additional","affiliation":[{"name":"Department of Hematology CHU Saint Etienne Saint Priest en Jarez F\u201042270 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Yves","family":"Cesbron","sequence":"additional","affiliation":[{"name":"Department of Immunology CHU Grenoble La Tronche F\u201038700 France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,6]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"311","article-title":"Imprecision of ratio\u2010derived differential leukocyte counts","volume":"11","author":"R\u00fcmke CL.","year":"1985","journal-title":"Blood Cells"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20457"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20529"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20382"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1002\/cyto.a.21105","article-title":"Leukoflow: Multiparameter extended white blood cell differentiation for routine analysis by flow cytometry","volume":"79","author":"Geijn GJ","year":"2011","journal-title":"Cytometry Part A"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20439"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21233"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20484"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.122"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22247"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.234"},{"key":"e_1_2_6_15_1","first-page":"1234","article-title":"The phenotype of normal, reactive and malignant plasma cells. 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