{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:17:10Z","timestamp":1789514230970,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T00:00:00Z","timestamp":1464048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Swedish Cancer Society (CF)"},{"name":"Swedish Heart and Lung Foundation"},{"name":"Swedish Pain Foundation (SSF)"},{"name":"Assar Gabrielsson's Cancer Research Foundation"},{"name":"LUA\/ALF Funding at the Sahlgrenska University Hospital"},{"name":"Swedish Research Council (Vetenskapsr\u00e5det)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The nucleosomal histone protein H2AX is specifically phosphorylated (\u03b3\u2010H2AX) adjacent to DNA double\u2010strand breaks (DSBs) and is used for quantifying DSBs. Many chemotherapies and ionizing radiation (IR) used in cancer treatment result in DSBs. Therefore, \u03b3\u2010H2AX has a significant potential as a biomarker in evaluating patient sensitivity and responsiveness to IR and chemotherapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, we report a flow cytometry\u2010based quantification of \u03b3\u2010H2AX (FCM\u2010\u03b3\u2010H2AX assay) customized for clinical practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We validated that our method is able to detect DNA damage in peripheral blood mononuclear cells (PBMCs) treated with DSB inducing agents. The method also detected the DNA repair deficiency in PBMCs treated with DNA repair inhibitors, as well as the deficiency in DNA repair signaling in PBMCs from two ataxia telangiectasia patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The FCM\u2010\u03b3\u2010H2AX assay has sufficient analytical sensitivity and precision to measure levels of DNA damage and DNA repair for clinical purposes. \u00a9 2016 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21374","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T14:57:04Z","timestamp":1460300224000},"page":"534-540","source":"Crossref","is-referenced-by-count":48,"title":["Validation of a flow cytometry\u2010based detection of \u03b3\u2010H2AX, to measure DNA damage for clinical applications"],"prefix":"10.1002","volume":"92","author":[{"given":"Pegah","family":"Johansson","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine, Sahlgrenska Academy at the University of Gothenburg Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Fasth","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Institute of Clinical Sciences, Sahlgrenska Academy at the University of Gothenburg Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torben","family":"Ek","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Hospital of Halland Halmstad Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ola","family":"Hammarsten","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine, Sahlgrenska Academy at the University of Gothenburg Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08467"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR3085.1"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19960601)77:11<2379::AID-CNCR29>3.0.CO;2-T"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1053-4296(05)80034-7"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mpo.10052"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1259\/bjr.20150172"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/1354750X.2014.898099"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2008.08.003"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2013.07.007"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/2041-9414-2-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semradonc.2010.05.004"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-200790"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgt270"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22512"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00247-014-2983-3"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.5772\/33257"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-009-0234-4"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005859"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.880385"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/RLI.0b013e3181a654a5"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1169"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113738"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.93"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874241601205010033"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR3003"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/258427a0"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20566"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3240341"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1053\/beha.2001.0158"}],"container-title":["Cytometry 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