{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T23:07:21Z","timestamp":1721603241426},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T00:00:00Z","timestamp":1479340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"New Hungary Development Plan, co-financed by the European Social Fund and the European Regional Development Fund","award":["T\u00c1MOP-4.2.2.A-11\/1\/KONV-2012-0045"],"award-info":[{"award-number":["T\u00c1MOP-4.2.2.A-11\/1\/KONV-2012-0045"]}]},{"name":"Lajos Szodoray Grant of the University of Debrecen"},{"name":"New National Excellence Program of the Ministry of Human Capacities"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Bronchoalveolar mesenchymal stem cells (MSCs) play an important role in the maintenance of lung integrity. Therapeutic application of bone marrow\u2010derived MSCs reduced chronic bronchial inflammation in idiopathic pulmonary fibrosis, and improved the ratio of survivors in sepsis with pneumonia. This study investigated the effect of MSCs from bronchoalveolar lavage fluid (BALF) of hypersensitivity pneumonitis (HP) on T\u2010cell function under <jats:italic>in vitro<\/jats:italic> conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bronchoalveolar MSCs were obtained via bronchoscopy with BAL from children with severe subacute HP. As control, BALF MSCs were assessed from children without any inflammatory lung disease. Isolated MSCs were characterized via immunophenotyping by flow cytometry and confocal laser scanning microscopy. HP\u2010derived and healthy separated peripheral blood mononuclear cells (PBMCs) were stimulated by 5 \u00b5g\/mL phytohemagglutinin in the presence of HP\u2010derived or control MSCs in 5\u2010day cultures. Proliferation and activation of T\u2010cells were characterized by the mean fluorescence intensity (MFI) of 5,6\u2010carboxyfluorescein\u2010diacetat succinimidyl ester (CFSE) and CD25, CD69 as well as HLA\u2010DR surface positivities, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HP\u2010derived MSCs showed significantly lower level of CD73, CD90, and CD105 expression compared to control MSCs in both flow cytometric and confocal microscopic experiments. MSCs from HP did not reduce T\u2010cell proliferation based on CFSE MFI values, while the level of CD25 expression on both control and HP\u2010derived CD4+ and CD8+ T\u2010cells was significantly reduced by normal MSCs, while HP\u2010derived MSCs did not have any significant effect. The level of other activation markers was not markedly modulated by MSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>BALF MSCs from HP are unable to downregulate the proliferation and activation of T\u2010cells that may support the development of recurrent intrapulmonary inflammation in HP. \u00a9 2016 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21490","type":"journal-article","created":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T09:23:02Z","timestamp":1479374582000},"page":"363-368","source":"Crossref","is-referenced-by-count":3,"title":["Impaired Immunosuppressive Effect of Bronchoalveolar Mesenchymal Stem Cells in Hypersensitivity Pneumonitis: Preliminary Findings"],"prefix":"10.1002","volume":"94","author":[{"given":"Enik\u0151","family":"Balogh","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, Faculty of Medicine University of Debrecen Debrecen 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