{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:46:02Z","timestamp":1790009162079,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T00:00:00Z","timestamp":1484870400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007208","name":"Vanderbilt-Ingram Cancer Center","doi-asserted-by":"publisher","award":["P30 CA68485"],"award-info":[{"award-number":["P30 CA68485"]}],"id":[{"id":"10.13039\/100007208","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007208","name":"Vanderbilt-Ingram Cancer Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007208","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Follicular lymphoma (FL) is an indolent non\u2010Hodgkin lymphoma that has a risk of transformation to more aggressive lymphoma. Relatively little is known about the nonmalignant B\u2010cell and T\u2010cell subset composition within the tumor microenvironment and whether altered phenotypes are associated with patterns of lymphoma B\u2010cell heterogeneity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two mass cytometry (CyTOF) panels were designed to immunophenotype B and T cells in FL tumors. Populations of malignant B cells, nonmalignant B cells, and T cells from each FL tumor were identified and their phenotypes compared to B and T cells from healthy human tonsillar tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Diversity in cellular phenotype between tumors was greater for the malignant B cells than for nonmalignant B or T cells. The malignant B\u2010cell population bore little phenotypic similarity to any healthy B\u2010cell subset, and unexpectedly clustered closer to na\u00efve B\u2010cell populations than GC B\u2010cell populations. Among the nonmalignant B cells within FL tumors, a significant lack of GC and plasmablast B cells was observed relative to tonsil controls. In contrast, nonmalignant T cells in FL tumors were present at levels similar to their cognate tonsillar T\u2010cell subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mass cytometry revealed that diverse HLA\u2010DR expression on FL cells within individual tumors contributed greatly to tumor heterogeneity. Both malignant and nonmalignant B cells in the tumor bore little phenotypic resemblance to healthy GC B cells despite the presence of T follicular helper cells in the tumor. These findings suggest that ongoing signaling interactions between malignant B cells and intra\u2010tumor T cells shape the tumor microenvironment. \u00a9 2016 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21498","type":"journal-article","created":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T05:17:00Z","timestamp":1481260620000},"page":"79-87","source":"Crossref","is-referenced-by-count":24,"title":["Mass Cytometry of Follicular Lymphoma Tumors Reveals Intrinsic Heterogeneity in Proteins Including HLA\u2010DR and a Deficit in Nonmalignant Plasmablast and Germinal Center B\u2010Cell Populations"],"prefix":"10.1002","volume":"92","author":[{"given":"Cara Ellen","family":"Wogsland","sequence":"first","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison Rae","family":"Greenplate","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arne","family":"Kolstad","sequence":"additional","affiliation":[{"name":"Department of Oncology Oslo University Hospital Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"June Helen","family":"Myklebust","sequence":"additional","affiliation":[{"name":"Department of Cancer Immunology Institute for Cancer Research, Oslo University Hospital Oslo Norway"},{"name":"Centre for Cancer Biomedicine, University of Oslo Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan Michael","family":"Irish","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology Vanderbilt University Medical Center Nashville Tennessee"},{"name":"Department of Cancer Biology Vanderbilt University Nashville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanutte","family":"Huse","sequence":"additional","affiliation":[{"name":"Department of Cancer Immunology Institute for Cancer Research, Oslo University Hospital Oslo Norway"},{"name":"Centre for Cancer Biomedicine, University of Oslo Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-006-0035-4"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041869"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198806233182502"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-03-272278"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-09-457283"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002057107"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20477"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2016.03.085"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.56"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.07.009"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500904"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.06.024"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.05.022"},{"key":"e_1_2_5_15_1","first-page":"239","article-title":"Differences in the germinal centres of palatine tonsils and lymph nodes","volume":"43","author":"Brachtel EF","year":"1996","journal-title":"Scand J Immunol"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-2-2"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3006"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.005"},{"key":"e_1_2_5_19_1","first-page":"1","article-title":"High\u2010dimensional single\u2010cell cancer biology","volume":"377","author":"Irish JM","year":"2014","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_5_20_1","article-title":"Single cell analysis of human tissues and solid tumors with mass cytometry","author":"Leelatian N","year":"2016","journal-title":"Cytometry B Clin Cytom"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-15-0213"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142956.cy1017s53"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2594"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1991"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2015.05.008"},{"key":"e_1_2_5_26_1","unstructured":"R Core Team(2013). 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