{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T05:10:20Z","timestamp":1719119420872},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T00:00:00Z","timestamp":1491350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Microscopic leukocyte differentials display many drawbacks. Several single 5 to 8\u2010color tubes using multiparameter flow cytometry (MFC) are able to provide extended differentials with sequential gating\u2010based analysis strategies. We investigated a new 5\u2010color MFC method to perform an extended 8\u2010part differential with a simplified gating strategy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Whole blood was stained with a combination of antibodies including HLA\u2010DR\u2010FITC\/CD19\u2010PE\/CD45\u2010ECD\/CD16\u2010PC5\u2009+\u2009CD71\u2010PC5\/CD5\u2010PC7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An original approach was developed to exclude debris and straightforwardly gate the cells to identify sixteen populations. Strong correlations were obtained with the analyzer for neutrophils, lymphocytes, monocytes, and eosinophils (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>&gt;0.9). Abnormal cells, such as immature granulocytes and blast cells were identified with a good sensitivity and excellent correlation against cytomorphologic review (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>=0.66 and 0.99, respectively). The choice of HLA\u2010DR and CD5 improved specificity for the identification of activated T\u2010lymphocytes and some lymphoid neoplastic cells, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Here a new cytometric differential is proposed with a robust gating strategy which may be used even by unskilled cytometrists and can be easily automated. \u00a9 2017 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21524","type":"journal-article","created":{"date-parts":[[2017,3,21]],"date-time":"2017-03-21T16:19:10Z","timestamp":1490113150000},"page":"498-507","source":"Crossref","is-referenced-by-count":3,"title":["A 5\u2010color flow cytometric method for extended 8\u2010part leukocyte differential"],"prefix":"10.1002","volume":"92","author":[{"given":"Julien","family":"Guy","sequence":"first","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, CHU de Dijon Dijon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orianne","family":"Wagner\u2010Ballon","sequence":"additional","affiliation":[{"name":"D\u00e9partement d'H\u00e9matologie et d'Immunologie Biologiques H\u00f4pital Henri Mondor, APHP, UPEC, INSERM U955 IMRB Cr\u00e9teil France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Pages","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, CHU de Dijon Dijon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Bailly","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, CHU de Dijon Dijon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Borgeot","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, CHU de Dijon Dijon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010C","family":"B\u00e9n\u00e9","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, CHU de Nantes Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Maynadi\u00e9","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, CHU de Dijon Dijon France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/97.3.345"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.5858\/2006-130-596-LPATRO"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2007.01002.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12044"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9681(75)90077-6"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20457"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20382"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20529"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1002\/cyto.a.21105","article-title":"Leukoflow: Multiparameter extended white blood cell differentiation for routine analysis by flow cytometry","volume":"79","author":"Geijn G\u2010J","year":"2011","journal-title":"Cytometry Part A"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20893"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.3343\/kjlm.2011.31.3.131"},{"key":"e_1_2_5_13_1","unstructured":"KoepkeJ VanAssendelftO BrindzaL.Reference Leukocyte (WBC) Differential Count (Proportional) and Evaluation of Instrumental Methods; Approved Standard 2nd ed. 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