{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,15]],"date-time":"2025-06-15T20:22:25Z","timestamp":1750018945886},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:p>Anterior mediastinal biopsies consisting predominantly of small lymphocytes can be a diagnostic challenge, especially in small core biopsies. In these cases, immunophenotyping is often employed using flow cytometry, and\/or immunohistochemistry. However, due the overlap in T\u2010cell phenotype between thymic neoplasm\/hyperplasia (THY), T\u2010lymphoblastic lymphoma (T\u2010LBL), and reactive lymph nodes (RLN), biopsies consisting predominantly of T\u2010cells may still be difficult to differentiate. Previous studies have shown a specific CD3\/bcl\u20102 staining pattern in thymic T cells of humans and mice using flow cytometry. However, the utility of this finding in distinguishing T\u2010cells of THY, T\u2010LBL, and RLN has not been carefully evaluated. Our findings show that the pattern of CD3\/bcl\u20102 expression in thymic T\u2010cells can be used to help diagnose anterior mediastinal biopsies, even when limited specimen is provided. \u00a9 2017 International Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.21541","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T01:12:44Z","timestamp":1500340364000},"page":"444-450","source":"Crossref","is-referenced-by-count":5,"title":["Bcl\u20102 maturation pattern in T\u2010cells distinguishes thymic neoplasm\/hyperplasia, T\u2010lymphoblastic lymphoma, and reactive lymph nodes"],"prefix":"10.1002","volume":"94","author":[{"given":"Nicholas","family":"Ward","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology William Beaumont Hospital Royal Oak MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junaid","family":"Baqai","sequence":"additional","affiliation":[{"name":"Pathology Associates of Central Illinois, Memorial Medical Center Springfield Ill"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Zehnpfennig","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology William Beaumont Hospital Royal Oak MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Fine","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology William Beaumont Hospital Royal Oak MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology William Beaumont Hospital Royal Oak MI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc D.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology William Beaumont Hospital Royal Oak MI"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60487-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10685"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.5.974"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1309\/K2FY1TED8GEGFLNG"},{"key":"e_1_2_6_6_1","first-page":"312","article-title":"The analogy in cell immunophenotype and parameters of cell cycle of ectopic thymus, normal thymus, and some acute lymphoblastic leukemia of T\u2010phenotype","volume":"49","author":"Babus\u00edkov\u00e1 O","year":"2002","journal-title":"Neoplasma"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13244-012-0201-0"},{"key":"e_1_2_6_8_1","article-title":"Pathology and Genetics of Head and Neck Tumours","author":"Barnes L","year":"2005","journal-title":"IARC Press;"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200212000-00008"},{"key":"e_1_2_6_10_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2008"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-11-551481"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11547-008-0233-1"},{"key":"e_1_2_6_13_1","first-page":"185","volume-title":"Flow Cytometry in Clinical Diagnosis","author":"Carey JL","year":"2007"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"2546","DOI":"10.4049\/jimmunol.151.5.2546","article-title":"Expression of the Bcl\u20102 protein in murine and human thymocytes and in peripheral T lymphocytes","volume":"15","author":"Veis DJ","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.5.1745"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21541","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21541","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T16:23:58Z","timestamp":1695486238000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21541"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1002\/cyto.b.21541"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21541","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8]]}}}