{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T10:56:35Z","timestamp":1769511395335,"version":"3.49.0"},"reference-count":11,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,15]],"date-time":"2017-11-15T00:00:00Z","timestamp":1510704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Oligoarticular juvenile idiopathic arthritis (oJIA) is the most frequent form of chronic arthritis in children; the clinical course is extremely variable. In this study we have characterized by flow cytometry synovial B and T cells subsets in patients with oJIA in order to identify any parameters that could predict a more aggressive course of disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>B and T cells from synovial fluid (SF) of 39 patients with oJIA were characterized by flow cytometry. In 22 patients SF was analysed at the onset of the disease (GroupA), in 17 SF was analysed at articular relapse (Group B). All patients in Group A were followed up for at least for 2 years after SF analysis: 13 patients relapsed during the follow\u2010up period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Comparison of SF from Group A and Group B demonstrated an activated phenotype in relapsed patients, with higher Switched Memory B cells (58.53 vs 36.07% of CD19+, <jats:italic>P<\/jats:italic>\u2010value 0.004) and lower Na\u00efve B cells (8.53 vs 25.9 of CD19+, <jats:italic>P<\/jats:italic>\u2010value 0.002) in Group B. Furthermore, patients from Group A who did not relapse showed lower percentages of synovial DNT (2.38 vs 1.50% of CD3\u2009+\u2009TCRalpha\/beta+, <jats:italic>P<\/jats:italic>\u2010value 0.025) and \u03b3\u03b4 T cells (19.1 vs 15.0% of CD3+ cells, <jats:italic>P<\/jats:italic>\u2010value 0.004) at the onset, if compared with other Group A patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In oJIA relapse SF present an activated B phenotype. Patients at disease onset with DNTs &lt;1.8% and\/or \u03b3\u03b4 T cells &lt;16% of CD3+ in synovial fluid have longer free\u2010disease survival.  \u00a9 2017 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21597","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T22:32:53Z","timestamp":1508797973000},"page":"423-427","source":"Crossref","is-referenced-by-count":5,"title":["Low synovial double negative T and \u03b3\u03b4 T cells predict longer free\u2010disease survival in oligoarticular JIA"],"prefix":"10.1002","volume":"94","author":[{"given":"Francesco","family":"Licciardi","sequence":"first","affiliation":[{"name":"Department of Pediatrics Citt\u00e0 della Salute e della Scienza, Regina Margherita Children Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Ceci","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Citt\u00e0 della Salute e della Scienza, Regina Margherita Children Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Toppino","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Citt\u00e0 della Salute e della Scienza, Regina Margherita Children Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Turco","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Citt\u00e0 della Salute e della Scienza, Regina Margherita Children Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Martino","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Citt\u00e0 della Salute e della Scienza, Regina Margherita Children Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuela","family":"Ricotti","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Citt\u00e0 della Salute e della Scienza, Regina Margherita Children Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Ferro","sequence":"additional","affiliation":[{"name":"Department of Immunohaematology Cardinal Massaia Hospital Asti Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Montin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Citt\u00e0 della Salute e della Scienza, Regina Margherita Children Hospital Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,15]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"390","article-title":"International League of Associations for Rheumatology classification of juvenile idiopathic arthritis: second revision, Edmonton, 2001","volume":"31","author":"Petty RE","year":"2004","journal-title":"J Rheumatol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh620"},{"key":"e_1_2_5_4_1","first-page":"S89","article-title":"Early predictors of outcome in juvenile idiopathic arthritis","volume":"21","author":"Ravelli A","year":"2003","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2014-205265"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200103223441207"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00245.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2824"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/erm.2013.14"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.20460"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040982"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2932531"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21597","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21597","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T16:23:53Z","timestamp":1695486233000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,15]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1002\/cyto.b.21597"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21597","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,15]]}}}