{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T14:15:52Z","timestamp":1781360152927,"version":"3.54.1"},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,11,16]],"date-time":"2017-11-16T00:00:00Z","timestamp":1510790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Detection and quantitation of fetomaternal hemorrhage (FMH) can be difficult in patients with pre\u2010existing elevations of HbF, such as those with hemoglobinopathies. The aim of this study was to evaluate the utility of dual\u2010color flow cytometry with the Fetal Cell Count Kit (FCCK) in differentiating adult and fetal HbF in this population, as compared to flow cytometry (FC) using HbF alone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood was obtained from normal adults and patients with hemoglobinopathies (\u03b2\u2010thalassemia and sickle cell disease), including a small number of pregnant females. Cord blood was used to spike some samples with 5% fetal cells. Analysis by single color (HbF) and dual\u2010color (HbF and carbonic anhydrase) FC was performed on these samples. Fetal cells were defined as those with high HbF fluorescence on single\u2010color FC, and those that were HbF\u2009+\u2009CA\u2212 using the FCCK. The quantity of fetal cells detected by each technique was compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty\u2010six adult patients were included. In non\u2010pregnant adults with hemoglobinopathies, a population of red cells with a fetal cell phenotype were detected by both techniques. The dual\u2010color method reported lower quantities of these cells. In nineteen samples spiked with cord blood the FCCK consistently underestimated the quantity of fetal cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Patients with \u03b2\u2010thalassemia and sickle cell disease have a population of HbF\u2010containing cells which are phenotypically similar to fetal cells. Even with dual\u2010color flow cytometry (FCCK), the detection and quantification of FMH by flow cytometry in this population remains difficult. \u00a9 2017 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21598","type":"journal-article","created":{"date-parts":[[2017,10,26]],"date-time":"2017-10-26T15:42:37Z","timestamp":1509032557000},"page":"695-698","source":"Crossref","is-referenced-by-count":8,"title":["The presence of F cells with a fetal phenotype in adults with hemoglobinopathies limits the utility of flow cytometry for quantitation of fetomaternal hemorrhage"],"prefix":"10.1002","volume":"94","author":[{"given":"Jad","family":"Othman","sequence":"first","affiliation":[{"name":"Institute of Haematology Royal Prince Alfred Hospital Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Orellana","sequence":"additional","affiliation":[{"name":"Institute of Haematology Royal Prince Alfred Hospital Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin Selina","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Haematology Royal Prince Alfred Hospital Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Russell","sequence":"additional","affiliation":[{"name":"Institute of Haematology Royal Prince Alfred Hospital Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teh\u2010Liane","family":"Khoo","sequence":"additional","affiliation":[{"name":"Institute of Haematology Royal Prince Alfred Hospital Camperdown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2012.01143.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1990.tb07848.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01271.x"},{"key":"e_1_2_5_5_1","unstructured":"Clinical and Laboratory Standards Institute. Red blood cell diagnostic testing using flow cytometry; approved guideline 2nd ed. CLSI document H52\u2010A2. Wayne PA: Clinical and Laboratory Standards Institute;2014."},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12518"},{"key":"e_1_2_5_7_1","first-page":"570","article-title":"The prevalence of maternal F cells in a pregnant population and potential overestimation of foeto\u2010maternal haemorrhage as a consequence","volume":"12","author":"Corcoran D","year":"2014","journal-title":"Blood Transfus"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15513815.2016.1209260"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03769.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12030"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2006.06.013"},{"key":"e_1_2_5_12_1","first-page":"462","article-title":"Experience in the evaluation of foeto\u2010maternal haemorrhage by flow cytometry","volume":"11","author":"Tazzari PL","year":"2013","journal-title":"Blood Transfus"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01780.x"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21598","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T15:30:29Z","timestamp":1694964629000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,16]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1002\/cyto.b.21598"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21598","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,16]]}}}