{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:02:39Z","timestamp":1790719359508,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,17]],"date-time":"2018-01-17T00:00:00Z","timestamp":1516147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["MZ CR VES16\u201032339A"],"award-info":[{"award-number":["MZ CR VES16\u201032339A"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The data on the clinical utility of the quantitative assessment of immunophenotypes in distinguishing mature CD5\u2010positive B\u2010cell neoplasms is limited. The study aim was to assess the diagnostic value of the quantitative assessment of a panel of 18 markers and to identify the most informative ones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The immunophenotype of the neoplastic population was determined in diagnostic specimens from 188 patients. BD FACSCanto II flow cytometer and FACSDiva software were used to analyze the positivity\/negativity and mean fluorescence intensity (MFI) of the surface expression of 18 markers. Advanced data mining methods were used to define the key differential diagnostic features of CLL\/SLL (chronic lymphocytic leukemia\/small lymphocytic lymphoma), MCL (mantle cell lymphoma), and CD5+ MZL (marginal zone lymphoma).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The most informative markers for the distinction of CLL\/SLL, MCL, CD5+ MZL, including atypical cases, were the MFI values of CD79b, CD20, CD23, CD43, CD38, CD11c, FMC7, CD200, kappa light chain, and their combinations. CD23 and CD200 were the most discriminant between CLL\/SLL and MCL and CD23 plus CD79b between CLL\/SLL and CD5+ MZL. The quantitative analysis of the most informative markers failed to accurately distinguish MCL and CD5+ MZL. The study highlights the data mining methods for the analysis and selection of the most informative immunophenotypic markers and for the design of a predictive model (diagnostic classifier), minimizing the subjectivity of expert\u2010based assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data confirmed that the quantification of the expression of informative markers increases the diagnostic value of immunophenotyping in mature CD5+ B\u2010cell neoplasms. \u00a9 2017 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21607","type":"journal-article","created":{"date-parts":[[2017,12,9]],"date-time":"2017-12-09T02:32:29Z","timestamp":1512786749000},"page":"576-587","source":"Crossref","is-referenced-by-count":17,"title":["Quantitative assessment of informative immunophenotypic markers increases the diagnostic value of immunophenotyping in mature CD5\u2010positive B\u2010cell neoplasms"],"prefix":"10.1002","volume":"94","author":[{"given":"David","family":"Starostka","sequence":"first","affiliation":[{"name":"Department of Clinical Haematology Hospital in Havirov  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Kriegova","sequence":"additional","affiliation":[{"name":"Department of Immunology Palacky University &amp; University Hospital Olomouc  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milos","family":"Kudelka","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Faculty of Electrical Engineering and Computer Science Technical University of Ostrava  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Mikula","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology Hospital in Havirov  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarka","family":"Zehnalova","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Faculty of Electrical Engineering and Computer Science Technical University of Ostrava  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Radvansky","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Faculty of Electrical Engineering and Computer Science Technical University of Ostrava  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Papajik","sequence":"additional","affiliation":[{"name":"Department of Haemato\u2010oncology Palacky University &amp; University Hospital Olomouc  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Kolacek","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology Hospital in Havirov  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Chasakova","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology Hospital in Havirov  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Talianova","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology Hospital in Havirov  Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,17]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow SH","year":"2008"},{"key":"e_1_2_7_3_1","article-title":"Quantitative flow cytometry: Concerns and recommendations in clinic and research","author":"Mizrahi O","year":"2017","journal-title":"Cytometry B Clin Cytom"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11242"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-62389-4_54"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","unstructured":"ZehnalovaS KudelkaM PlatosJ HorakZ.Local representatives in weighted networks. IEEE\/ACM International Conference on Advances in Social Networks Analysis and Mining (ASONAM 2014). IEEE2014;870\u2013875.","DOI":"10.1109\/ASONAM.2014.6921688"},{"key":"e_1_2_7_7_1","first-page":"18","article-title":"Classification and regression by randomForest","volume":"2","author":"Liaw A","year":"2002","journal-title":"R News"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20363"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20010215)46:1<23::AID-CYTO1033>3.0.CO;2-Z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21065"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/cytob.21128","article-title":"CD200 has an important role in the differential diagnosis of mature B\u2010cell neoplasms by multiparameter flow cytometry","volume":"86","author":"Sandes AF","year":"2014","journal-title":"Cytometry Part B"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP59UORCYZEVQO"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2009.01.017"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V124.21.5637.5637"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP38XRRUGSQOVC"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.10101"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.5858\/2006-130-1850-FCAOLC","article-title":"Flow cytometric analysis of lymphomas: current status and usefulness","volume":"130","author":"Kaleem Z.","year":"2006","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4065\/83.7.776"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402584"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2007.08.002"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPV8LN5ENMZOVY"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.10045"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1309\/VWTK-XYT5-D0DK-06HQ"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1309\/UQ4N-M5KL-0ANY-YD3G"},{"key":"e_1_2_7_26_1","first-page":"1140","article-title":"Quantitative expression of CD23 and its ligand CD21 in chronic lymphocytic leukemia","volume":"85","author":"Lopez\u2010Matas M","year":"2000","journal-title":"Haematologica"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2405068"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.011049"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20426"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPOGCI3DAXVUMI"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2015.01.002"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12356"},{"key":"e_1_2_7_33_1","article-title":"Expression of CD43 in chronic lymphoproliferative leukemias","author":"Sorigue M","year":"2017","journal-title":"Cytometry Part B"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.2310\/JIM.0b013e31823908f9"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1309\/TE56-A43X-29TT-5H8G"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2012.32.1.95"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3960\/jslrt.52.185"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1309\/UD2C6JVPWHXQQ217"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1309\/U98ADVKUC26R2RJA"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2008.03060.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184059"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nicl.2017.08.002"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2017.1349332"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12911-017-0518-1"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genhosppsych.2017.03.001"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbi.2017.08.005"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2017.07.012"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2017.00398"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06516-1"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.05.031"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2017.05.009"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16-21053"},{"key":"e_1_2_7_53_1","first-page":"185","article-title":"Comparing the efficacy of surgery and medical therapy for pain management in endometriosis: a systematic review and meta\u2010analysis","volume":"20","author":"Chaichian S","year":"2017","journal-title":"Pain Phys"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.56"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21607","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21607","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T15:30:08Z","timestamp":1694964608000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,17]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1002\/cyto.b.21607"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21607","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,17]]}}}