{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T23:07:41Z","timestamp":1781305661002,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,23]],"date-time":"2018-01-23T00:00:00Z","timestamp":1516665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Multiparametric flow cytometry is a useful tool for diagnosis of plasma cell (PC) dyscrasias and assessment of minimal residual disease in plasma cell myeloma (PCM). However, the immunophenotypic differences between the clonal PCs of PCM and those of monoclonal gammopathy of undetermined significance (MGUS) as well as the correlation of these flow cytometric markers with pertinent laboratory parameters have not been evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively identified all newly diagnosed treatment\u2010naive PCM and MGUS patients between 09\/2014 and 06\/2015 who underwent 10\u2010color flow\u2010cytometric evaluation: CD45, CD38, CD138, cKappa, cLambda, CD19, CD27, CD28, CD56, CD117. FACSDiva analysis was used to identify antigenic aberrancies and associations with pertinent laboratory parameters were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All cases demonstrated at least two aberrancies. There was a trend toward a greater number of aberrancies in PCM, with 68% showing &gt;\/= 4 aberrancies compared with 44% in MGUS (<jats:italic>P<\/jats:italic>\u2009=\u20090.11). The only marker more frequently aberrant in one disease class was CD19, aberrant in 68% of PCM and 25% of MGUS (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). In PCM, significant associations were found for CD56 non\u2010aberrancy (<jats:italic>P<\/jats:italic>\u2009=\u20090.05) and the presence of amyloid and CD27 aberrancy and normal serum albumin (<jats:italic>P<\/jats:italic>\u2009=\u20090.05). In MGUS, CD117 expression was associated with normal hemoglobin (<jats:italic>P<\/jats:italic>\u2009=\u20090.03).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The PCs of PCM show a trend toward more antigenic aberrancy than those of MGUS. There is significant association between the antigenic profiles of PCM\/MGUS and clinical parameters including amyloidosis, albumin level, and hemoglobin. \u00a9 2018 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21624","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T04:19:59Z","timestamp":1515557999000},"page":"500-508","source":"Crossref","is-referenced-by-count":7,"title":["Flow cytometric aberrancies in plasma cell myeloma and MGUS \u2013 correlation with laboratory parameters"],"prefix":"10.1002","volume":"94","author":[{"given":"Sarika","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Pathology University of Iowa Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitin J.","family":"Karandikar","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Iowa Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Ginader","sequence":"additional","affiliation":[{"name":"Holden Comprehensive Cancer Center University of Iowa Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8264-6379","authenticated-orcid":false,"given":"Andrew M.","family":"Bellizzi","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Iowa Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol J.","family":"Holman","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Iowa Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21332"},{"key":"e_1_2_6_3_1","first-page":"2197","article-title":"Multiple myeloma","volume":"385","author":"R\u00f6llig C","journal-title":"Lancet 2015;"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1011442"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2011.63"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70442-5"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-1038"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-505487"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa01133202"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.20.6.1625"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-05-088443"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.189"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404284"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-11-568907"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21265"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2013.04.008"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(00)00418-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08121.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.009100"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11080"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.5858\/2007-131-951-QOPCIB"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2013.12.007"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07929.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-05-221689"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.15.4120"},{"key":"e_1_2_6_26_1","first-page":"1655","article-title":"Immunophenotypic characterization of plasma cells from monoclonal gammopathy of undetermined significance patients. 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