{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:53:07Z","timestamp":1790833987474,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>V\u03b41<jats:sup>+<\/jats:sup> T cells, a subset of \u03b3\u03b4 T cells, are responsible for innate\u2010like immune responses. Recently, an anti\u2010tumor function mediated by MHC\u2010unrestricted recognition of lipid and stress molecules, has also been described in these cells. This study aimed to quantify and phenotypically characterize circulating V\u03b41<jats:sup>+<\/jats:sup> T cells in B cell Chronic Lymphocytic Leukemia (CLL) and Monoclonal B cell lymphocytosis (MBL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study enrolled 58 individuals distributed in five groups: Binet B and C CLL (<jats:italic>n<\/jats:italic>\u2009=\u20099), Binet A CLL (<jats:italic>n<\/jats:italic>\u2009=\u200926), High count\u2010MBL (<jats:italic>n<\/jats:italic>\u2009=\u200910), Low count\u2010MBL (<jats:italic>n<\/jats:italic>\u2009=\u20095), and a control group (<jats:italic>n<\/jats:italic>\u2009=\u20098). The phenotypic characterization of V\u03b41<jats:sup>+<\/jats:sup> T cells, as well as the other T cell subpopulations (CD4<jats:sup>+<\/jats:sup>, CD8<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>, and V\u03b41<jats:sup>\u2212<\/jats:sup> \u03b3\u03b4T cells), were assessed by flow cytometry, evaluating the frequency of each subset expressing CD27, CD69, and cytoplasmic granzyme B.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed an increasing percentage of V\u03b41<jats:sup>+<\/jats:sup> T cells belonging to CD27<jats:sup>\u2212<\/jats:sup> compartment from controls to advanced stages of the disease, which was accompanied by an increasing percentage of these cells expressing granzyme B, a phenotypic pattern that was also observed in the other T cell subpopulations under study since earlier stages of the disease. Moreover, a striking expansion of V\u03b41<jats:sup>+<\/jats:sup> T cells in Binet B and C CLL was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These experiment findings point to an expansion of CD27<jats:sup>\u2010<\/jats:sup>V\u03b41<jats:sup>+<\/jats:sup> T cells with a cytotoxic profile, from controls to advanced stages of the disease, which points to a role of V\u03b41<jats:sup>+<\/jats:sup> T cells in the host's anti\u2010tumor responses against clonal B\u2010cells in MBL and CLL. \u00a9 2018 Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21645","type":"journal-article","created":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T06:14:46Z","timestamp":1539843286000},"page":"164-168","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Quantification and phenotypic characterization of peripheral blood V\u03b41\u2009+\u2009T cells in chronic lymphocytic leukemia and monoclonal B cell lymphocytosis"],"prefix":"10.1002","volume":"96","author":[{"given":"Catarina","family":"Sim\u00f5es","sequence":"first","affiliation":[{"name":"Unidade de Gest\u00e3o Operacional de Citometria, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"},{"name":"Departamento de Qu\u00edmica Universidade de Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Silva","sequence":"additional","affiliation":[{"name":"Unidade de Gest\u00e3o Operacional de Citometria, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anabela","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Unidade de Gest\u00e3o Operacional de Citometria, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Silva","sequence":"additional","affiliation":[{"name":"Unidade de Gest\u00e3o Operacional de Citometria, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Santos","sequence":"additional","affiliation":[{"name":"Unidade de Gest\u00e3o Operacional de Citometria, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"Marques","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Patologia Cl\u00ednica, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Hematologia Cl\u00ednica, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Roque","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Hematologia Cl\u00ednica, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Carda","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Hematologia Cl\u00ednica, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"},{"name":"Coimbra Institute for Clinical and Biomedical Research, Faculdade de Medicina  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.B.","family":"Sarmento\u2010Ribeiro","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Hematologia Cl\u00ednica, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"},{"name":"Coimbra Institute for Clinical and Biomedical Research, Faculdade de Medicina  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria do Ros\u00e1rio","family":"Domingues","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Universidade de Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Hematologia Cl\u00ednica, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Paiva","sequence":"additional","affiliation":[{"name":"Unidade de Gest\u00e3o Operacional de Citometria, Centro Hospitalar e Universit\u00e1rio de Coimbra  Coimbra Portugal"},{"name":"Coimbra Institute for Clinical and Biomedical Research, Faculdade de Medicina  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv303"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06867.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"e_1_2_6_5_1","first-page":"387","article-title":"Evaluation of CD307a expression patterns during normal B\u2010cell maturation and in B\u2010cell malignancies by flow cytometry","volume":"94","author":"Auat M","year":"2018","journal-title":"Cytom Part B Clin Cytom"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2014.104"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3384"},{"key":"e_1_2_6_8_1","first-page":"755","article-title":"Targeting gamma delta T cells for cancer immunotherapy: Bench to bedside","volume":"138","author":"Gogoi D","year":"2013","journal-title":"Indian J Med Res"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3904"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-0597"},{"key":"e_1_2_6_11_1","first-page":"86","article-title":"Molecular diversity of gammadelta T cells in peripheral blood from patients with B\u2010cell chronic lymphocytic leukaemia","volume":"49","author":"Bartkowiak J","year":"2002","journal-title":"Neoplasma"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-2417"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653249.2011.553595"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20545"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2010.503820"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"40558","DOI":"10.18632\/oncotarget.9941","article-title":"Phenotypic alteration of CD8\u2009+\u2009T cells in chronic lymphocytic leukemia is associated with epigenetic reprogramming","volume":"7","author":"Wu J","year":"2016","journal-title":"Oncotarget"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2012.39"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21645","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21645","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21645","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T07:53:55Z","timestamp":1694418835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,17]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/cyto.b.21645"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21645","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,17]]},"assertion":[{"value":"2018-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}