{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T08:57:33Z","timestamp":1775984253799,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Feng R: National Natural Science Foundation for Young Scholars of China","award":["81600132"],"award-info":[{"award-number":["81600132"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of this study was to determine whether immunophenotypic profiles detected by flow cytometry are useful in differentiating chronic myelomonocytic leukemia (CMML) from reactive monocytosis, and between CMML subtypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eight\u2010color flow cytometry was used to immunophenotype blasts, monocytes, and granulocytes in the bone marrow of 34 patients with CMML and 12 patients with reactive monocytosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bone marrow myeloblast, promonocyte, and monocyte counts by flow cytometry were significantly higher in the CMML group than in the reactive monocytosis group. Myeloblast aberrancies were present in all CMML patients as compared with 2 of 12 (16.7%) reactive monocytosis patients (<jats:italic>P<\/jats:italic> &lt; 0.001). The number of blast aberrancies ranged from one to nine (median, four) in CMML patients and 94.1% of CMML cases exhibited \u2265 two aberrancies. In contrast, two reactive monocytosis cases showed only one phenotypic abnormality of blasts. Monocyte and granulocyte aberrancies were present in 26 of 34 (76.5%) and in 31 of 34 (91.2%) CMML patients, respectively. Decreased side scatter (SSC) and abnormal CD11b\/CD13\/CD16 maturation pattern in granulocytes were more frequent in CMML than in reactive monocytosis. No significant differences in antigen expression were detected between the CMML subtypes except that altered CD45\/SSC pattern on the blasts was more commonly observed in CMML\u20100\/1 than in CMML\u20102.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CMML has phenotypic aberrancies in monocytes, granulocytes, and more frequently in myeloblasts. Aberrant expression of two or more antigens in myeloblasts by flow cytometry has a high sensitivity (94.1%) and a high specificity (100%) to differentiate CMML from reactive monocytosis. \u00a9 2018 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21721","type":"journal-article","created":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T06:16:40Z","timestamp":1539843400000},"page":"901-909","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Application of immunophenotypic analysis in distinguishing chronic myelomonocytic leukemia from reactive monocytosis"],"prefix":"10.1002","volume":"94","author":[{"given":"Ru","family":"Feng","sequence":"first","affiliation":[{"name":"Departments of Hematology Beijing Hospital, National Center of Gerontology  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijaya Raj","family":"Bhatt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Hematology and Oncology University of Nebraska Medical Center  Omaha Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology University of Nebraska Medical Center  Omaha Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Pirruccello","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology University of Nebraska Medical Center  Omaha Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology University of Nebraska Medical Center  Omaha Nebraska"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,17]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"82","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid tissues","author":"Swerdlow S","year":"2017"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.119"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1182\/blood.V78.1.205.205","article-title":"Philadelphia\u2010negative (Ph\u2010) chronic myeloid leukemia (CML): Comparison with Ph+ CML and chronic myelomonocytic leukemia. 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