{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:48:02Z","timestamp":1792702082205,"version":"4.5.0"},"reference-count":117,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T00:00:00Z","timestamp":1540166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>Hodgkin's lymphoma (HL) has a unique immunophenotype derived from immunohistochemistry (positive for CD15, CD30, and Pax\u20105; negative for CD3, CD20 in most cases, and CD45). The knowledge gained over recent years enables better diagnosis, prognosis, and treatment of HL. Flow cytometry as a tool for the diagnosis of classic HL has not been useful in the past due to the difficulty in isolating Reed\u2013Sternberg cells as they are admixed in a rich inflammatory background which consists mainly of T cells, B cells, eosinophils, histiocytes, and plasma cells. However, in the recent past, several studies have tried to identify Reed\u2013Sternberg cells using flow cytometry on fine needle aspiration or tissue biopsy of lymph nodes to confirm or supplement immunohistochemistry staining in diagnosis. Newer and more sensitive tools such as flow cytometry can be used for diagnosis, technology that may have been difficult in the past for diagnosis of this lymphoma subtype. Using flow cytometry, diagnosis is faster and could lead to point\u2010of\u2010care technology especially where we have typical immunophenotype signatures. \u00a9 2018 International Clinical Cytometry Society<\/jats:p>","DOI":"10.1002\/cyto.b.21724","type":"journal-article","created":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T03:40:04Z","timestamp":1540266004000},"page":"116-127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Use of flow cytometry in the phenotypic diagnosis of hodgkin's lymphoma"],"prefix":"10.1002","volume":"96","author":[{"given":"Ravnit\u2010Kaur","family":"Grewal","sequence":"first","affiliation":[{"name":"MBCHB South African National Bioinformatics Institute, University of the Western Cape  Bellville South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manogari","family":"Chetty","sequence":"additional","affiliation":[{"name":"Department of Oral and Molecular Biology, Faculty of Dentistry University of the Western Cape  Cape Town South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel\u2010Akinola","family":"Abayomi","sequence":"additional","affiliation":[{"name":"Global Emerging Pathogens Treatment Consortium  Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciprian","family":"Tomuleasa","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Research Center for Functional Genomics and Translational Medicine Iuliu Hatieganu University of Medicine and Pharmacy\u2014Ion Chiricuta Oncology Institute  Cluj Napoca Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan R.","family":"Fromm","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Washington  Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/095952873201700106"},{"key":"e_1_2_7_3_1","first-page":"56","article-title":"Cases of enlargement of the lymphatics glands and spleen (or, Hodgkin's disease) with remarks","volume":"11","author":"Wilks S","year":"1856","journal-title":"Guys Hosp Rep"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2005.05.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2460"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14518"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2016.10.007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2016.05.009"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2014.01.006"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31822832de"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2004.10.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2004.12.006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17122127"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2013.02.010"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21799"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2359"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24272"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2016-204065"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2016.10.008"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30473"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.177"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-293050"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2012.11.003"},{"issue":"7","key":"e_1_2_7_25_1","first-page":"772","article-title":"Lymphocyte\u2010depleted classical Hodgkin lymphoma accompanied by myelofibrosis","volume":"58","author":"Sakatoku K","year":"2017","journal-title":"Rinsho Ketsueki"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2015-203145"},{"issue":"1","key":"e_1_2_7_27_1","first-page":"69","article-title":"B\u2010cell transcription factors Pax\u20105, Oct\u20102, BOB.1, Bcl\u20106, and MUM1 are useful markers for the diagnosis of nodular lymphocyte predominant Hodgkin lymphoma","volume":"52","author":"Herbeck R","year":"2011","journal-title":"Rom J Morphol Embryol"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800622"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-0867"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-229583"},{"key":"e_1_2_7_31_1","first-page":"7531729","article-title":"PAX5\u2010negative classical Hodgkin lymphoma: A case report of a rare entity and review of the literature","volume":"2017","author":"Vali Betts E","year":"2017","journal-title":"Case Rep Hematol"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205629"},{"issue":"8","key":"e_1_2_7_33_1","first-page":"XC04","article-title":"Diagnostic utility of PAX5 in Hodgkin and non\u2010Hodgkin lymphoma: A study from northern India","volume":"10","author":"Johri N","year":"2016","journal-title":"J Clin Diagn Res"},{"issue":"6","key":"e_1_2_7_34_1","first-page":"383","article-title":"Expression of TIA1 and PAX5 in classical Hodgkin lymphoma at initial diagnosis may predict clinical outcome","volume":"24","author":"Nguyen TT","year":"2016","journal-title":"AIMM"},{"key":"e_1_2_7_35_1","first-page":"523","article-title":"WHO classification of lymphomas: Implications for clinical practice and translational research","volume":"2009","author":"The JES","year":"2008","journal-title":"Hematology Am Soc Hematol Educ Program"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880289"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2009.1.491"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2017.03.001"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.08.015"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3765"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-08-2577"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-08-2525"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209982"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209954"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401838"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2017.04.007"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207410"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-11-4019"},{"issue":"1","key":"e_1_2_7_49_1","first-page":"3","article-title":"Can Reed\u2013Sternberg cells of classic Hodgkin's disease be considered B\u2010cells?","volume":"3","author":"Carbone A","year":"1999","journal-title":"Adv Clin Pathol"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24502"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07969"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090528"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2009.02.007"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2009.01300.x"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1309\/0Q7F-QTGM-6DPD-TLGY"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwn058"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-168096"},{"issue":"3","key":"e_1_2_7_58_1","first-page":"253","article-title":"Immune reactions in classical Hodgkin's lymphoma","volume":"36","author":"Poppema S","year":"1999","journal-title":"Semin Hematol"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65424-7"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.2000.0327"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/5.suppl_1.S121"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-05-024109"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190600580973"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138416"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5556"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1309\/7371XK6F6P7474XX"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPW3UN9DYLDSPB"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21261"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPY8E2LYHCGUFP"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP0Q1SVOXBHMAM"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21117"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-269-8_2"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20561"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20535"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474086.2017.1318053"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/387034"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20407"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20376"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190802308728"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31817d7452"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-73-6-881"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197507)36:1<252::AID-CNCR2820360128>3.0.CO;2-7"},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/7.suppl_4.S105"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.43.4803"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.131.6.1280202"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1100340"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.62.4817"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-05-285379"},{"key":"e_1_2_7_89_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1111961"},{"key":"e_1_2_7_90_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1000067"},{"key":"e_1_2_7_91_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.11.6525"},{"key":"e_1_2_7_92_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-007-0356-9"},{"key":"e_1_2_7_93_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1506340"},{"key":"e_1_2_7_94_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1408648"},{"key":"e_1_2_7_95_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2013.51.9298"},{"key":"e_1_2_7_96_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S133385"},{"key":"e_1_2_7_97_1","doi-asserted-by":"publisher","DOI":"10.1080\/17474086.2017.1350167"},{"key":"e_1_2_7_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(15)70013-6"},{"key":"e_1_2_7_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)60165-9"},{"key":"e_1_2_7_100_1","doi-asserted-by":"publisher","DOI":"10.5045\/br.2017.52.3.159"},{"key":"e_1_2_7_101_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408363.2015.1030063"},{"key":"e_1_2_7_102_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-703470"},{"key":"e_1_2_7_103_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.66.4482"},{"key":"e_1_2_7_104_1","doi-asserted-by":"publisher","DOI":"10.1006\/smim.1998.0156"},{"key":"e_1_2_7_105_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(16)30167-X"},{"key":"e_1_2_7_106_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-06-644336"},{"key":"e_1_2_7_107_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2014.1.151"},{"key":"e_1_2_7_108_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon-2016-0160"},{"key":"e_1_2_7_109_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.12911"},{"key":"e_1_2_7_110_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.67.3467"},{"key":"e_1_2_7_111_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-06-361055"},{"key":"e_1_2_7_112_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14133"},{"key":"e_1_2_7_113_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.321"},{"key":"e_1_2_7_114_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.60.4363"},{"key":"e_1_2_7_115_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.38.1350"},{"key":"e_1_2_7_116_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2015.10.015"},{"key":"e_1_2_7_117_1","doi-asserted-by":"publisher","DOI":"10.1159\/000443469"},{"key":"e_1_2_7_118_1","doi-asserted-by":"publisher","DOI":"10.1515\/rjim-2016-0021"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21724","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21724","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21724","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21724","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T07:53:44Z","timestamp":1694418824000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21724"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,22]]},"references-count":117,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/cyto.b.21724"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21724","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,22]]},"assertion":[{"value":"2018-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}