{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T10:16:00Z","timestamp":1783764960973,"version":"3.55.0"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T00:00:00Z","timestamp":1539648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Laborat\u00f3rio Sabin"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The cytometric flow osmotic fragility test (FC\u2010OFT) was recently introduced. However, the test is still under development and some variables have not yet been fully tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The osmotic fragility of hereditary spherocytosis (HS) cases and healthy controls were evaluated by FC\u2010OFT using a series of tubes containing decreasing concentrations of NaCl. The analyses were executed in fresh and incubated (37\u00b0C for 24 h) blood samples anticoagulated with EDTA and heparin. The percentages of residual red blood cells were used to plot the osmotic fragility curves. The OF curves of each tested condition were compared using the median corpuscular fragility (MCF). ROC curve analyses identified the most accurate NaCl concentrations for differentiation between HS cases and healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>FC\u2010OFT curves assumed a sigmoidal dose\u2013response shape and the MCF of cases and controls were different in all instances. MCF comparisons revealed that incubation and anticoagulant have major and minor effects on the FC\u2010OFT, respectively. One hundred percent of sensitivity and specificity was obtained from 5.5 to 6.0 g\/L of NaCl in EDTA\u2010treated fresh blood, from 6.0 to 8.0 g\/L of NaCl in EDTA\u2010treated incubated blood, and in none of the tested NaCl concentration in heparinized blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>EDTA is the anticoagulant of choice for the assay. Incubation at 37\u00b0C for 24 h increased its diagnostic capability. The most reliable NaCl concentration for the discrimination of HS case from controls was 6.0 g\/L of NaCL in fresh EDTA\u2010treated blood, and was 7.5 g\/L of NaCl in incubated EDTA\u2010treated blood. \u00a9 2018 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21733","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T06:12:12Z","timestamp":1539756732000},"page":"910-917","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Flow Cytometric Analysis of Erythrocytes Osmotic Fragility in Hereditary Spherocytosis: A Case\u2010Controlled Study Evaluating the Best Anticoagulant, Sample Pre\u2010Treatment and NaCl Concentration for Reliable Screening of this Red Blood Cell Membrane Disorder"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2354-3417","authenticated-orcid":false,"given":"Camila Santos","family":"Nobre","sequence":"first","affiliation":[{"name":"Sabin Laboratory  Bras\u00edlia Federal District Brazil"},{"name":"Post\u2010Graduation of Health Science University of Bras\u00edlia  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeovania Almeida","family":"Silva","sequence":"additional","affiliation":[{"name":"Sabin Laboratory  Bras\u00edlia Federal District Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Henriques","family":"J\u00e1como","sequence":"additional","affiliation":[{"name":"Sabin Laboratory  Bras\u00edlia Federal District Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00eddia Freire Abdalla","family":"Nery","sequence":"additional","affiliation":[{"name":"Sabin Laboratory  Bras\u00edlia Federal District Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2136-3471","authenticated-orcid":false,"given":"Gustavo Barcelos","family":"Barra","sequence":"additional","affiliation":[{"name":"Sabin Laboratory  Bras\u00edlia Federal District Brazil"},{"name":"Post\u2010Graduation of Health Science University of Bras\u00edlia  Bras\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(96)90027-X"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12335"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08921.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1002\/cyto.b.21511","article-title":"Flow cytometric osmotic fragility test: Increased assay sensitivity for clinical application in pediatric hematology","volume":"94","author":"Ciepiela O","year":"2017","journal-title":"Cytometry B Clin Cytom"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2000.063"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05052.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20448"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21143"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20583"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2014-3516"},{"key":"e_1_2_7_12_1","first-page":"245","volume-title":"The Hemolytic Anemias","author":"Dacie J","year":"1985"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.1998.00014.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI101847"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2011.01380.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1586\/ehm.11.51"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-90-481-9485-8_22"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21733","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21733","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21733","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21733","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T06:51:07Z","timestamp":1694760667000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21733"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,16]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/cyto.b.21733"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21733","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,16]]},"assertion":[{"value":"2018-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}