{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:51:41Z","timestamp":1793278301467,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T00:00:00Z","timestamp":1539648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Mycosis fungoides (MF) and S\u00e9zary Syndrome (SS) are clinically distinct cutaneous T\u2010cell lymphomas with strikingly similar morphologic and phenotypic features. Prior studies have suggested phenotypic differences based on markers of antigen experience, suggesting a different cell of origin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seventy\u2010nine involved peripheral blood or bone marrow samples from 33 patients with SS and 19 patients with MF were studied by 10\u2010color flow cytometry, including CD62L, CD45RA, CCR4, and PD\u20101. Gated tumor events were classified as na\u00efve (<jats:italic>T<\/jats:italic><jats:sub>N<\/jats:sub>), central memory (<jats:italic>T<\/jats:italic><jats:sub>CM<\/jats:sub>), effector memory (<jats:italic>T<\/jats:italic><jats:sub>EM<\/jats:sub>), or effector memory with reacquired CD45RA (<jats:italic>T<\/jats:italic><jats:sub>EMRA<\/jats:sub>); based on CD62L<jats:sup>+<\/jats:sup>\/CD45RA<jats:sup>+<\/jats:sup>, CD62L<jats:sup>+<\/jats:sup>\/CD45RA<jats:sup>\u2212<\/jats:sup>, CD62L<jats:sup>\u2212<\/jats:sup>\/CD45RA<jats:sup>\u2212<\/jats:sup>, or CD62L<jats:sup>\u2212<\/jats:sup>\/CD45RA<jats:sup>+<\/jats:sup> phenotype, respectively. Sequential specimens were compared to assess for phenotypic stability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The na\u00efve\/memory phenotype of the neoplastic T\u2010cells was markedly heterogeneous, with a dominant <jats:italic>T<\/jats:italic><jats:sub>N<\/jats:sub>, <jats:italic>T<\/jats:italic><jats:sub>CM<\/jats:sub>, <jats:italic>T<\/jats:italic><jats:sub>EM<\/jats:sub>, or <jats:italic>T<\/jats:italic><jats:sub>EMRA<\/jats:sub> subset on 11 (14%), 32 (41%), 30 (38%), and 6 (8%) cases, respectively. There was no correlation between the diagnosis of MF or SS and putative cell of origin (<jats:italic>P<\/jats:italic> = 0.4). Overexpression of CCR4 and PD1 was observed in most cases, with higher intensity in SS compared to MF. The na\u00efve\/memory phenotype remained the same for 10 patients up to 273 days after the initial analysis; while on six patients, the na\u00efve\/memory phenotype was different from the original phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both SS and MF can have phenotypic features of any of the major na\u00efve\/memory T\u2010cell subsets, which questions the current principle of \u201ccell\u2010of\u2010origin\u201d distinction between SS and MF. Phenotypic shifts within these subsets are common, suggesting a functional state rather than a cell\u2010of\u2010origin surrogate. \u00a9 2018 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21738","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T06:12:43Z","timestamp":1539756763000},"page":"234-241","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Na\u00efve\/memory T\u2010cell phenotypes in leukemic cutaneous T\u2010cell lymphoma: Putative cell of origin overlaps disease classification"],"prefix":"10.1002","volume":"96","author":[{"given":"Pedro","family":"Horna","sequence":"first","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn C.","family":"Moscinski","sequence":"additional","affiliation":[{"name":"Department of Hematopathology and Laboratory Medicine, H. Lee Moffitt Cancer Center  Tampa Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubomir","family":"Sokol","sequence":"additional","affiliation":[{"name":"Department of Malignant Hematology H. Lee Moffitt Cancer Center  Tampa Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7207-731X","authenticated-orcid":false,"given":"Haipeng","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Hematopathology and Laboratory Medicine, H. 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