{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:38:49Z","timestamp":1792640329307,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,18]],"date-time":"2018-11-18T00:00:00Z","timestamp":1542499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>While it is known that CD123 is normally strongly expressed on plasmacytoid dendritic cells and completely absent on nucleated red blood cells, detailed information regarding CD123 expression in acute leukemia is scarce and, if available, hard to compare due to different methodologies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>CD123 expression was evaluated using standardized EuroFlow immunophenotyping in 139 pediatric AML, 316 adult AML, 193 pediatric BCP\u2010ALL, 69 adult BCP\u2010ALL, 101 pediatric T\u2010ALL, and 28 adult T\u2010ALL patients. Paired diagnosis\u2010relapse samples were available for 57 AML and 19 BCP\u2010ALL patients. Leukemic stem cell (LSC) data was available for 32 pediatric AML patients. CD123 expression was evaluated based on mean fluorescence intensity, median fluorescence intensity, and percentage CD123 positive cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>EuroFlow panels were stable over time and between laboratories. CD123 was expressed in the majority of AML and BCP\u2010ALL patients, but absent in most T\u2010ALL patients. Within AML, CD123 expression was lower in erythroid\/megakaryocytic leukemia, higher in NPM1 mutated and FLT3\u2010ITD mutated leukemia, and comparable between LSC and leukemic blasts. Within BCP\u2010ALL, CD123 expression was higher in patients with (high) hyperdiploid karyotypes and the BCR\u2010ABL fusion gene. Interestingly, CD123 expression was increased in BCP\u2010ALL relapses while highly variable in AML relapses (compared to CD123 expression at diagnosis).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Authors evaluated CD123 expression in a large cohort of acute leukemia patients, based on standardized and reproducible methodology. Our results may facilitate stratification of patients most likely to respond to CD123 targeted therapies and serve as reference for CD123 expression (in health and disease). \u00a9 2018 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cyto.b.21745","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T02:17:13Z","timestamp":1542593833000},"page":"134-142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":119,"title":["CD123 expression levels in 846 acute leukemia patients based on standardized immunophenotyping"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5094-7700","authenticated-orcid":false,"given":"Anne E.","family":"Bras","sequence":"first","affiliation":[{"name":"Laboratory Medical immunology (LMI), Department of Immunology, Erasmus MC University Medical Center Rotterdam  Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"de Haas","sequence":"additional","affiliation":[{"name":"Dutch Childhood Oncology Group  the Hague the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"van Stigt","sequence":"additional","affiliation":[{"name":"Laboratory for Translational Immunology (LTI) University Medical Center Utrecht  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojca","family":"Jongen\u2010Lavrencic","sequence":"additional","affiliation":[{"name":"Department of Hematology, Erasmus MC University Medical Center Rotterdam  Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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