{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T17:46:37Z","timestamp":1697132797401},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,18]],"date-time":"2018-11-18T00:00:00Z","timestamp":1542499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Neurotuberculosis is one of the commonest HIV associated opportunistic infections of the central nervous system in India. HIV\u2010TB coinfection may lead to altered frequencies of T cells, thereby influencing the course and progression of the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined the frequencies of T cell subsets in HIV infected individuals with neurotuberculosis (HIV+nTB+) as compared to individuals with HIV associated systemic TB (HIV+sTB+), asymptomatic HIV (HIV+TB\u2010), non\u2010HIV neuro TB (HIV\u2010nTB+), non\u2010HIV systemic TB (HIV\u2010sTB+), and healthy controls (HIV\u2010TB\u2010). Activation and senescence profiles of CD4 and CD8 T cells and memory subsets in peripheral blood mononuclear cells were studied by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The significant observations among the T cell subsets in HIV+nTB+ were: (1) <jats:italic>Na\u00efve T cells<\/jats:italic>: decreased CD4 T cells compared to HIV\u2010sTB+ (<jats:italic>P<\/jats:italic> = 0.005); decreased CD8 T cells compared to HIV\u2010nTB+ and HIV\u2010TB\u2010 (<jats:italic>P<\/jats:italic> \u2264 0.007), (2) <jats:italic>Memory T cells<\/jats:italic>: expanded CD4 TEMRA cells compared to HIV\u2010nTB+, HIV\u2010sTB+, and HIV\u2010TB\u2010 (<jats:italic>P<\/jats:italic> \u2264 0.003); expanded CD8 TEMRA cells compared to HIV\u2010nTB+ and HIV\u2010TB\u2010 (<jats:italic>P<\/jats:italic> \u2264 0.005), (3) <jats:italic>Activated T cells<\/jats:italic>: higher CD4 T cells compared to HIV\u2010nTB+, HIV\u2010sTB+, and HIV\u2010TB\u2010 (<jats:italic>P<\/jats:italic> \u2264 0.004); higher CD8 T cells compared to HIV + TB\u2010, HIV\u2010nTB+, HIV\u2010sTB+, and HIV\u2010TB\u2010 (<jats:italic>P<\/jats:italic> \u2264 0.001), and (4) <jats:italic>Senescent T cells<\/jats:italic>: increased CD8 T cells compared to HIV\u2010nTB+ and HIV\u2010TB\u2010 groups (<jats:italic>P<\/jats:italic> = 0.000).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Increased activation compared to HIV+TB\u2010, HIV\u2010nTB+, HIV\u2010sTB+, and HIV\u2010TB\u2010 groups and increased senescence compared to HIV\u2010nTB+ and HIV\u2010TB\u2010 groups were observed in CD8 T cells in HIV+nTB+, suggesting that the frequencies of these T cell subsets are altered to a greater extent in these individuals. \u00a9 2018 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21746","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T07:21:36Z","timestamp":1542612096000},"page":"270-281","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Alteration of T Cell Phenotypes in HIV\u2010Neurotuberculosis Coinfection"],"prefix":"10.1002","volume":"98","author":[{"given":"Deepashri","family":"Rao","sequence":"first","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences (NIMHANS)  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manjunatha M.","family":"Venkataswamy","sequence":"additional","affiliation":[{"name":"Department of Neurovirology National Institute of Mental Health and Neurosciences (NIMHANS)  Bangalore 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