{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:20:00Z","timestamp":1790742000919,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,3]],"date-time":"2019-02-03T00:00:00Z","timestamp":1549152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006694","name":"Associazione Italiana Contro le Leucemie - Linfomi e Mieloma","doi-asserted-by":"publisher","award":["Sezione Siena"],"award-info":[{"award-number":["Sezione Siena"]}],"id":[{"id":"10.13039\/501100006694","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["IG 20133"],"award-info":[{"award-number":["IG 20133"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Recent investigations in chronic myeloid leukemia (CML) have focused on the identification and characterization of leukemic stem cells (LSCs). These cells reside within the CD34<jats:sup>+<\/jats:sup>\/CD38<jats:sup>\u2500<\/jats:sup>\/Lin<jats:sup>\u2500<\/jats:sup> fraction and score positive for CD26 (dipeptidylpeptidase IV) a marker, expressed in both bone marrow (BM) and peripheral blood (PB) samples, that discriminates CML cells from normal hematopoietic stem cells (HSCs) or from LSCs of other myeloid neoplasms. CD26 evaluation could be a useful tool to improve the identification of CML LCSs by using flow\u2010cytometry assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>CD26<jats:sup>+<\/jats:sup> LSCs have been isolated from EDTA PB and BM samples of patients with leucocytosis suspected for CML. Analysis of LSCs CML has been performed by using custom\u2010made lyophilized pre\u2010titrated antibody mixture test and control tube and a CD45<jats:sup>+<\/jats:sup>\/CD34<jats:sup>+<\/jats:sup>\/CD38<jats:sup>\u2212<\/jats:sup>\/CD26<jats:sup>+<\/jats:sup> panel as a strict flow cytometric gating strategy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of CD26 on CD34<jats:sup>+<\/jats:sup>\/CD38<jats:sup>\u2212<\/jats:sup> population was detectable in 211\/211 PB and 84\/84 BM samples of subsequently confirmed BCR\u2010ABL<jats:sup>+<\/jats:sup> CP\u2010CML patients. None of the 32 samples suspicious for CML but scoring negative for circulating CD26<jats:sup>+<\/jats:sup> LSCs were diagnosed as CML after conventional cytogenetic and molecular testing. To validate our results, we checked for PB CD26<jats:sup>+<\/jats:sup> LSCs in patients affected by other hematological disorders and they all scored negative for CD26 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We propose flow cytometry evaluation of CD26 expression on PB CD34<jats:sup>+<\/jats:sup>\/CD38<jats:sup>\u2212<\/jats:sup> population as a new rapid, reproducible, and powerful diagnostic tool for the diagnosis of CML. \u00a9 2019 The Authors. <jats:italic>Cytometry Part B: Clinical Cytometry<\/jats:italic> published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21764","type":"journal-article","created":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T04:44:29Z","timestamp":1549255469000},"page":"294-299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Flow Cytometry Assessment of CD26<sup>+<\/sup> Leukemic Stem Cells in Peripheral Blood: A Simple and Rapid New Diagnostic Tool for Chronic Myeloid Leukemia"],"prefix":"10.1002","volume":"96","author":[{"given":"Donatella","family":"Raspadori","sequence":"first","affiliation":[{"name":"Hematology Unit Azienda Ospedaliera Universitaria Senese  Siena Italy"},{"name":"Hematology Unit University of Siena and Azienda Ospedaliera Universitaria Senese  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Pacelli","sequence":"additional","affiliation":[{"name":"Hematology Unit University of Siena and Azienda Ospedaliera Universitaria Senese  Siena Italy"},{"name":"Department of Medical Biotechnologies University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sicuranza","sequence":"additional","affiliation":[{"name":"Hematology Unit University of Siena and Azienda Ospedaliera Universitaria Senese  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Abruzzese","sequence":"additional","affiliation":[{"name":"Division of Hematology Ospedale S. Eugenio  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Iurlo","sequence":"additional","affiliation":[{"name":"Hematology Division, Foundation IRCCS Ca' Granda\u2010Ospedale Maggiore Policlinico University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Cattaneo","sequence":"additional","affiliation":[{"name":"Hematology Division, Foundation IRCCS Ca' Granda\u2010Ospedale Maggiore Policlinico University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Gozzini","sequence":"additional","affiliation":[{"name":"Hematology Unit Careggi University Hospital (AOUC) Firenze  Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Galimberti","sequence":"additional","affiliation":[{"name":"Department of Hematology, Clinical and Experimental Medicine University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Barat\u00e8","sequence":"additional","affiliation":[{"name":"Department of Hematology, Clinical and Experimental Medicine University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Pregno","sequence":"additional","affiliation":[{"name":"Hematology Division Azienda Ospedaliera Citt\u00e0 della Salute e della Scienza  Torino Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maura","family":"Nicolosi","sequence":"additional","affiliation":[{"name":"Hematology Division Azienda Ospedaliera Citt\u00e0 della Salute e della Scienza  Torino Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Sor\u00e0","sequence":"additional","affiliation":[{"name":"Universit\u00e0 Cattolica del Sacro Cuore Sede di Roma  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Annunziata","sequence":"additional","affiliation":[{"name":"Hematology Cardarelli Hospital Napoli  Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigiana","family":"Luciano","sequence":"additional","affiliation":[{"name":"Hematology Federico II University  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Caocci","sequence":"additional","affiliation":[{"name":"Hematology Unit, Department of Medical Sciences University of Cagliari  Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Moretti","sequence":"additional","affiliation":[{"name":"Azienda USL Toscana Centro Ospedale San Giovanni di Dio  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Sgherza","sequence":"additional","affiliation":[{"name":"Division of Hematology Casa Sollievo Sofferenza  San Giovanni Rotondo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Fozza","sequence":"additional","affiliation":[{"name":"Department of Medical, Surgical and Experimental Sciences University of Sassari  Sassari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"Russo","sequence":"additional","affiliation":[{"name":"Hematology Unit, AOU G. Martino  Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"Usala","sequence":"additional","affiliation":[{"name":"Hematology Unit Ospedale Oncologico A. Businco  Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina A.","family":"Liberati","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera S. Maria Division of Onco\u2010Hematology  Terni Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Ciofini","sequence":"additional","affiliation":[{"name":"Hematology Unit University of Siena and Azienda Ospedaliera Universitaria Senese  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika M.","family":"Trawinska","sequence":"additional","affiliation":[{"name":"Division of Hematology Ospedale S. 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