{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T15:23:06Z","timestamp":1765380186821},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T00:00:00Z","timestamp":1549497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>High\u2010grade B\u2010cell lymphomas (HGBCL) with <jats:italic>MYC<\/jats:italic> and <jats:italic>BCL2<\/jats:italic> or\/and <jats:italic>BCL6<\/jats:italic> rearrangements (R), so\u2010called double\/triple\u2010hit lymphomas (DH\/THL), are uncommon, clinically aggressive lymphomas that require a prompt diagnosis. We aim to identify flow cytometric immunophenotypic (IP) features of DH\/THL that may aid in triaging these cases followed by a timely confirmatory cytogenetic study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared the IP features of 43 cases of DH\/THL to those of 55 cases of single\u2010hit lymphoma (SHL) and 59 cases of diffuse large B\u2010cell lymphoma (DLBCL) without <jats:italic>MYC<\/jats:italic>\u2010R (<jats:italic>MYC<\/jats:italic><jats:sup>neg<\/jats:sup> DLBCL). We analyzed the expression patterns of CD10, CD19, CD20, CD38, and surface immunoglobulin light chain in lymphoma cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bright CD38 expression (CD38<jats:sup>bright<\/jats:sup>) analyzed either qualitatively or semi\u2010quantitatively was more common in DH\/THL (56%) than in <jats:italic>MYC<\/jats:italic><jats:sup>neg<\/jats:sup> DLBCL (17%) but less common compared to SHL (82%), indicating that CD38<jats:sup>bright<\/jats:sup> can serve as a biomarker for DH\/THL. Additionally, CD38<jats:sup>bright<\/jats:sup> may be a better indicator for predicting DH\/THL\u2010<jats:italic>BCL2<\/jats:italic> than DHL\u2010<jats:italic>BCL6<\/jats:italic>, and very bright CD38 expression was exclusive to <jats:italic>MYC<\/jats:italic> rearranged lymphomas. The expression patterns of other markers were similar among these lymphoma groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CD38<jats:sup>bright<\/jats:sup> is a biomarker associated with DH\/THL with a moderate sensitivity (~50%) and high specificity (~90%). While this marker cannot be used as a screening tool, awareness of this correlation may aid in expediting the diagnosis and prioritizing FISH testing in resource limited settings or situations when samples are limited. Future studies to combine immunohistochemical markers are needed to further enhance the predictive power of CD38<jats:sup>bright<\/jats:sup> in diagnosing DH\/THL. \u00a9 2019 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21770","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T05:05:30Z","timestamp":1549602330000},"page":"368-374","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Bright CD38 Expression by Flow Cytometric Analysis Is a Biomarker for Double\/Triple Hit Lymphomas with a Moderate Sensitivity and High Specificity"],"prefix":"10.1002","volume":"96","author":[{"given":"Abdullah","family":"Alsuwaidan","sequence":"first","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaina","family":"Pirruccello","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesse","family":"Jaso","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasad","family":"Koduru","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolando","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JoEllen","family":"Krueger","sequence":"additional","affiliation":[{"name":"Medical City at Dallas  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meggie","family":"Doucet","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahman","family":"Chaudhry","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin","family":"Fuda","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weina","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Texas Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,7]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"335","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Kluin PM","year":"2017"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-212191"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2014.1.107"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2014.1.90"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-02-636316"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-03-836361"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPL11MAHISIJBQ"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21076"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP7YLDTJPLCE5F"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190902930470"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.76"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2013.879714"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1309\/6UGDU03VVD0L98LQ"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31826cebad"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21227"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-12-820605"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21770","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21770","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21770","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21770","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T18:46:21Z","timestamp":1694285181000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,7]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1002\/cyto.b.21770"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21770","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,7]]},"assertion":[{"value":"2018-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}