{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T05:39:23Z","timestamp":1757309963657,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T00:00:00Z","timestamp":1554768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Compelling evidence has emerged for the relevance of flow cytometry (FC) in the diagnostic work\u2010up of myelodysplastic syndromes (MDS) but due to technical issues, the erythroid lineage has been under investigated, specifically in the therapeutic context.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using the \u201cno red cell lysis\u201d method developed to set up the RED\u2010score, we specifically quantified the fraction of CD117\/c\u2010KIT\u2010expressing erythroid precursors in a cohort of 144 MDS patients and studied the correlation with response to erythropoiesis\u2010stimulating agents (ESA) in a sub cohort of 63 low\u2010risk MDS patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We confirmed the previously reported increase in CD117\/c\u2010KIT\u2010expressing erythroid precursors in a subset of MDS patients and demonstrated a strong association between a cut off of CD117\/c\u2010KIT\u2010expressing erythroid precursors \u22653% and ESA response (<jats:italic>P<\/jats:italic>\u2009=\u20090.001), independent of red blood cell requirement. From our observations, we hypothesized that a decrease in CD117\/c\u2010KIT\u2010expressing erythroid precursors could be a mechanism of ESA failure. Moreover, the fraction of CD117\/c\u2010KIT\u2010expressing erythroid precursors was correlated with progression\u2010free survival in low\u2010risk MDS patients (<jats:italic>P<\/jats:italic>\u2009=\u20090.018). In vitro, we demonstrated in an EPO dependent cell line that CD117\/c\u2010KIT expression is necessary for cell survival under EPO stimulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The quantification of the CD117\/c\u2010KIT\u2010expressing erythroid precursors could be proposed as a new theranostic and prognostic marker in MDS treated by ESA. Future studies will be required to determine whether modulating CD117\/c\u2010KIT expression and signaling could be used to improve anemia in MDS. \u00a9 2019 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21781","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T08:29:15Z","timestamp":1554798555000},"page":"215-222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The fraction of CD117\/c\u2010KIT\u2010expressing erythroid precursors predicts ESA response in low\u2010risk myelodysplastic syndromes"],"prefix":"10.1002","volume":"96","author":[{"given":"Anna","family":"Raimbault","sequence":"first","affiliation":[{"name":"Service d'H\u00e9matologie biologique H\u00f4pitaux Universitaires Paris Centre\u2010Cochin  Paris France"},{"name":"INSERM U1016, UMR 8104, Universit\u00e9 Paris Descartes H\u00f4pitaux Universitaires Paris Centre  Paris 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