{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:06:39Z","timestamp":1790370399358,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,4,11]],"date-time":"2019-04-11T00:00:00Z","timestamp":1554940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The diagnosis of T\u2010cell neoplasms is often challenging, due to overlapping features with reactive T\u2010cells and limitations of currently available T\u2010cell clonality assays. The description of an antibody specific for one of two mutually exclusive T\u2010cell receptor (TCR) \u03b2\u2010chain constant regions (TRBC1) provide an opportunity to facilitate the detection of clonal TCR\u03b1\u03b2 T\u2010cells based on TRBC\u2010restriction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty patients with mature T\u2010cell neoplasms and 44 patients without evidence of T\u2010cell neoplasia were studied. Peripheral blood (51), bone marrow (10), and lymph node (3) specimens were evaluated by 9\u2010color flow cytometry including TRBC1 (CD2\/CD3\/CD4\/CD5\/CD7\/CD8\/CD45\/TCR\u03b3\u03b4\/TRBC1 and\/or CD2\/CD3\/CD4\/CD5\/CD7\/CD8\/CD26\/CD45\/TRBC1). Monophasic TRBC1 expression on any immunophenotypically distinct CD4\u2010positive or CD8\u2010positive\/TCR\u03b3\u03b4\u2010negative T\u2010cell subset was considered indicative of clonality.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Monophasic (clonal) TRBC1 expression was identified on immunophenotypically abnormal T\u2010cells from all 20 patients with T\u2010cell malignancies (100% sensitivity), including 17 cases with either &gt;97% or &lt;3% TRBC1\u2010positive events, and three cases with monophasic homogenous TRBC1\u2010dim expression. All immunophenotypically distinct CD4\u2010positive and CD8\u2010positive\/TCR\u03b3\u03b4\u2010negative T\u2010cell subsets from 44 patients without T\u2010cell malignancies showed the expected mixture of TRBC1\u2010positive and TRBC\u20101\u2010negative subpopulations (non\u2010clonal), except for seven patients (16%) with very small CD8\u2010positive T\u2010cell subsets exhibiting a monophasic (clonal) pattern.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Inclusion of a single anti\u2010TRBC1 antibody into a diagnostic T\u2010cell flow cytometry panel facilitates the rapid identification of T\u2010cell neoplasms, in addition to small monotypic CD8\u2010positive subsets of uncertain significance. \u00a9 2019 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21782","type":"journal-article","created":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T01:51:05Z","timestamp":1555033865000},"page":"99-107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Single Antibody Detection of T\u2010Cell Receptor \u03b1\u03b2 Clonality by Flow Cytometry Rapidly Identifies Mature T\u2010Cell Neoplasms and Monotypic Small CD8\u2010Positive Subsets of Uncertain Significance"],"prefix":"10.1002","volume":"98","author":[{"given":"Min","family":"Shi","sequence":"first","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan","family":"Jevremovic","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory E.","family":"Otteson","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael M.","family":"Timm","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5783-4485","authenticated-orcid":false,"given":"Horatiu","family":"Olteanu","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Horna","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester 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