{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T19:38:43Z","timestamp":1783107523997,"version":"3.54.6"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T00:00:00Z","timestamp":1556841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Histological evaluation of malignant hematologic involvement of the skin can be challenging and needs an extended immunohistochemistry panel. We assessed the ability of flow cytometry (FCM) to detect neoplastic cell subsets in skin biopsies as a useful tool that supplements the histological examination in a complementary way.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two hundred and forty\u2010three consecutive skin biopsies were retrospectively analyzed between April 2012 and July 2017.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among them, 147 samples, corresponding to 128 patients, were analyzed at diagnosis. Eighty\u2010seven patients had erythrodermic inflammatory dermatoses, and 41 patients had cutaneous hematologic neoplasms. Cutaneous T\u2010cell lymphomas were the most frequent disorders, accounting for 70% of cases (29\/41). Cutaneous B\u2010cell lymphoma was found in only 17% of cases (7\/41) and immature hematologic malignancies in 5% (2\/41). Three patients had secondary skin involvement. The sensitivity of FCM skin biopsy analysis was 78.1% (32\/41). Among the 243 samples, 27 patients had mycosis fungoides (MF) or Sezary syndrome (SS) with available FCM data. A loss of CD26 expression was identified in 92% of cases of transformed MF or SS versus 40% of cases of non\u2010transformed MF (<jats:italic>P<\/jats:italic> = 0.0057 <jats:italic>\u03c7<\/jats:italic>\u00b2). Among the 12 MF patients with negative CD26 expression, six progressed to SS versus none in the positive group (50% vs. 0% <jats:italic>P<\/jats:italic> = 0.0168 <jats:italic>\u03c7<\/jats:italic>\u00b2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>FCM analysis of the skin biopsies is a sensitive method and a useful tool for improving the sensitivity of diagnosis of hematologic skin neoplasms. Among the MF patients, a loss of CD26 expression could be a marker of higher risk of progression. \u00a9 2019 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21784","type":"journal-article","created":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T07:30:36Z","timestamp":1556868636000},"page":"283-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Usefulness of Flow Cytometry for the Detection of Cutaneous Localization in Malignant Hematologic Disorders"],"prefix":"10.1002","volume":"96","author":[{"given":"Elsa","family":"Maitre","sequence":"first","affiliation":[{"name":"Laboratoire d'h\u00e9matologie, CHU de Caen, Calvados, 14033 Caen  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Laure","family":"Le\u2010Page","sequence":"additional","affiliation":[{"name":"Laboratoire d'Anatomopathologie, CHU de Caen, Calvados, 14033 Caen  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