{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T19:21:11Z","timestamp":1772738471867,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T00:00:00Z","timestamp":1563321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30900534"],"award-info":[{"award-number":["30900534"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81272626"],"award-info":[{"award-number":["81272626"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Extranodal natural killer (NK)\/T\u2010cell lymphoma, nasal type (ENKTL\u2010N) is an aggressive lymphoma typically diagnosed by examining small biopsy specimens. Flow cytometry is very valuable for the diagnosis and classification of several kinds of hematolymphoid neoplasms but has not been widely used for diagnosing ENKTL\u2010N.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We systematically investigated the flow cytometry characteristics of 26 solid tissue biopsy specimens of ENKTL\u2010N at initial diagnosis and compared the results with those from reactive NK\u2010cells in the nasal\/nasopharyngeal region and peripheral blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our study revealed seven flow cytometry (FCM)\u2010based characteristics for distinguishing between the neoplastic cells and reactive NK\u2010cells, including (1) the proportion of NK\u2010cells among total lymphocytes &gt;10%; (2) forward scatter &gt;10<jats:sup>5<\/jats:sup>; (3) mean fluorescence intensity of CD56\u2009&gt;\u20095,000; (4) aberrant antigen expression or loss; (5) skewed killer cell immunoglobulin\u2010like receptor repertoire; (6) homogenously positive for CD38; and (7) positive for CD30 or CD336. FCM\u2010based immunophenotyping is a potentially feasible and convenient approach for discriminating cellular lineages, evaluating the activation status of NK\u2010cells, and selecting potential therapy targets of ENKTL\u2010N.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Flow cytometry is very valuable for facilitating routine diagnosis, confirming clonality, predicting the cellular lineage, and guiding individual treatment for ENKTL\u2010N. \u00a9 2019 International Clinical Cytometry Society<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21838","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T09:35:30Z","timestamp":1563356130000},"page":"28-35","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comprehensive Flow\u2010Cytometry\u2010Based Immunophenotyping Analysis for Accurate Diagnosis and Management of Extranodal NK\/T Cell Lymphoma, Nasal Type"],"prefix":"10.1002","volume":"98","author":[{"given":"Jian\u2010Chao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology West China Hospital of Sichuan University  Chengdu China"},{"name":"Department of Pathology Fujian Cancer Hospital &amp; Fujian Medical University Cancer Hospital  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Qin","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital of Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital of Sichuan University  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Min","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital of Sichuan University  Chengdu 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