{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:17:08Z","timestamp":1787825828237,"version":"build-2784847793"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["EEQ\/2017\/000115 (Ver\u20101)"],"award-info":[{"award-number":["EEQ\/2017\/000115 (Ver\u20101)"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Red cell membranopathies refers to phenotypically and morphologically heterogeneous disorders. High throughput imaging flow cytometry (IFC) combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy to produce high content image analysis with quantitative analysis. We have evaluated the applications of IFC to examine both the morphology as well as fluorescence signal intensity in red cell membranopathies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fluorescence intensity of eosin\u20105\u2010maleimide (EMA) labeled red cells was measured for diagnosis of RBC membrane protein defect on Amnis ImageStreamX followed by Image analysis on IDEAS software to study features such as circularity and shape ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The hereditary spherocytosis (HS) group showed significantly decreased MFI (52,800\u2009\u00b1\u20099,100) than normal controls (81,100\u2009\u00b1\u20094,700) (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) whereas non\u2010HS showed 78,300\u2009\u00b1\u20099,900. The shape ratio of hereditary elliptocytosis (HE) was significantly higher (43.8%) than normal controls (14.6%). The circularity score is higher in HS (64.15%) than the normal controls (44.3%) whereas the circularity score was very less in HE (10%) due to the presence of elliptocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The advantages of the IFC over standard flow cytometry is its ability to provide high\u2010content image analysis and measurement of parameters such as circularity and shape ratio allow discriminating red cell membranopathies (HS and HE) due to variations in shape and size. It could be a single, effective, and rapid IFC test for detection and differentiation of red cell membrane disorders in hematology laboratories where an IFC is available.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21857","type":"journal-article","created":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T12:44:44Z","timestamp":1574340284000},"page":"238-249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Applications of imaging flow cytometry in the diagnostic assessment of red cell membrane disorders"],"prefix":"10.1002","volume":"98","author":[{"given":"Tejashree Anil","family":"More","sequence":"first","affiliation":[{"name":"Department of Hematogenetics ICMR\u2010National Institute of Immunohematology, KEM Hospital Campus  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhavik","family":"Dalal","sequence":"additional","affiliation":[{"name":"Department of Hematogenetics ICMR\u2010National Institute of Immunohematology, KEM Hospital Campus  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rati","family":"Devendra","sequence":"additional","affiliation":[{"name":"Department of Hematogenetics ICMR\u2010National Institute of Immunohematology, KEM Hospital Campus  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prashant","family":"Warang","sequence":"additional","affiliation":[{"name":"Department of Hematogenetics ICMR\u2010National Institute of Immunohematology, KEM Hospital Campus  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruna","family":"Shankarkumar","sequence":"additional","affiliation":[{"name":"Department of Hematogenetics ICMR\u2010National Institute of Immunohematology, KEM Hospital Campus  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3509-2913","authenticated-orcid":false,"given":"Prabhakar","family":"Kedar","sequence":"additional","affiliation":[{"name":"Department of Hematogenetics ICMR\u2010National Institute of Immunohematology, KEM Hospital Campus  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,21]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Image data exploration and analysis software user's manual","author":"Amnis Corporation","year":"2009"},{"key":"e_1_2_8_3_1","first-page":"274","article-title":"Hereditary red cell membrane defects: Diagnostic and clinical aspects","volume":"9","author":"Barcellini W.","year":"2011","journal-title":"Blood Transfusion"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2007.05.008"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23699"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/beha.2000.0081"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1988.tb00828.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00488a012"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(96)90027-X"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2013.04.003"},{"key":"e_1_2_8_11_1","volume-title":"Practical hematology","author":"Dacie J. V.","year":"2006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2006.03.005"},{"issue":"1","key":"e_1_2_8_13_1","first-page":"9","article-title":"The binding of eosin\u20105\u2032\u2010maleimide to red cells from patients with hereditary spherocytosis (Abstract)","volume":"117","author":"Fisher J.","year":"2002","journal-title":"British Journal of Haematology"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2013.09.001"},{"key":"e_1_2_8_15_1","first-page":"746","article-title":"Glycerol lysis time of incubated erythrocytes in the diagnosis of hereditary spherocytosis","volume":"84","author":"Gottfried E. L.","year":"1974","journal-title":"Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.06.023"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1109\/TSMC.1973.4309314"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.09.007"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2008.01098.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0141-9854.2003.00557.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02416.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04730.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20413"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12070"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(89)80794-2"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12746"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.04.009"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.12657"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22438"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00014a045"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI101847"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61588-3"},{"key":"e_1_2_8_33_1","first-page":"100761F\u20101","article-title":"Application of image flow cytometry for the characterization of red blood cell morphology","volume":"10076","author":"Pinto R. N.","year":"2017","journal-title":"Proceedings of Society of Photo\u2010Optical Instrumentation Engineers"},{"key":"e_1_2_8_34_1","first-page":"001","article-title":"Quantitative image analysis of cellular morphology using Amnis\u00ae ImageStreamX mark II imaging flow cytometer: A comparison against conventional methods","volume":"1","author":"Poh S.","year":"2015","journal-title":"Single Cell Biology"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00164"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21088"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.03.019"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-12-512772"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1159\/000094679"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1999.01130.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01779-14"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20583"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1980.tb07167.x"},{"key":"e_1_2_8_44_1","first-page":"279","article-title":"The ImageStream system: A key step to a new era in imaging","volume":"54","author":"Zuba\u2010Surma E. K.","year":"2007","journal-title":"Folia Histochemica et Cytobiologica"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-374912-3.00008-0"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21857","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21857","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21857","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T05:52:53Z","timestamp":1693979573000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21857"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,21]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1002\/cyto.b.21857"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21857","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,21]]},"assertion":[{"value":"2019-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}