{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:56:50Z","timestamp":1791345410663,"version":"4.3.1"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Differential diagnosis between diffuse large B\u2010cell lymphoma (DLBCL) and follicular lymphoma (FL) becomes a challenge when adequate biopsy is unavailable. The present study aimed to investigate the applicability of DNA cell cycle analysis by flow cytometry (FC) for differentiating between CD10<jats:sup>+<\/jats:sup> DLBCL and FL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Data were collected from 57 specimens where CD5<jats:sup>\u2212<\/jats:sup>\/CD10<jats:sup>+<\/jats:sup>\/light chain restricted B cells were detected. DNA staining was performed using the Coulter DNA Prep Kit. Cell cycle fractions were evaluated by automatic analysis using the ModFit LT software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histopathological analysis confirmed the diagnosis of CD10<jats:sup>+<\/jats:sup> FL in 30 specimens (52.6%), CD10<jats:sup>+<\/jats:sup> DLBCL in 24 specimens (42.1%), and CD10<jats:sup>+<\/jats:sup> Burkitt lymphoma in 3 specimens (5.3%). A significantly higher rate of DNA aneuploidy was detected among CD10<jats:sup>+<\/jats:sup> DLBCL than FL specimens (50 vs. 13.3% respectively, <jats:italic>p<\/jats:italic> =\u2009.003). Likewise, DNA index was significantly higher in CD10<jats:sup>+<\/jats:sup> DLBCL relative to FL (1.26\u2009\u00b1\u20090.35 vs. 1.04\u2009\u00b1\u20090.16 respectively, <jats:italic>p<\/jats:italic> =\u2009.004). Notably, the proportion of cells in the S\u2010phase and proliferative fraction was significantly higher in CD10<jats:sup>+<\/jats:sup> DLBCL than in CD10<jats:sup>+<\/jats:sup> FL (S\u2010phase: 15.97\u2009\u00b1\u200913.94 vs. 4.43\u2009\u00b1\u20094.41 mean\u2009\u00b1 <jats:italic>SD<\/jats:italic>, respectively, <jats:italic>p<\/jats:italic> &lt;\u2009.0001; proliferative fraction: 18.87\u2009\u00b1\u200915.17 vs. 5.78\u2009\u00b1\u20097.04 mean\u2009\u00b1 <jats:italic>SD<\/jats:italic>, respectively, <jats:italic>p<\/jats:italic> =\u2009.0001). Using a receiver operating characteristic analysis, optimal cutoffs for S\u2010phase \u22657% and proliferative fraction \u22659% were determined. These values could be used to differentiate between CD10<jats:sup>+<\/jats:sup> DLBCL and CD10<jats:sup>+<\/jats:sup> FL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Including DNA cell cycle analysis in the FC lymphoma assessment panel may be of diagnostic value in differentiating between CD10+ DLBCL and FL when adequate biopsy is unavailable.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21861","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T13:33:28Z","timestamp":1575898408000},"page":"449-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Flow cytometry aneuploidy and cell cycle indexing as a possible tool for differentiating between CD10<sup>+<\/sup> diffuse large B\u2010cell lymphoma and follicular lymphoma"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3451-5737","authenticated-orcid":false,"given":"David","family":"Azoulay","sequence":"first","affiliation":[{"name":"Azrieli Faculty of Medicine Bar\u2010Ilan University  Safed Israel"},{"name":"Hematology Unit and Laboratories Galilee Medical Center  Naharia Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector I.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Pathology Unit, Galilee Medical Center  Naharia Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene","family":"Dementiev","sequence":"additional","affiliation":[{"name":"Pathology Unit, Galilee Medical Center  Naharia Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Eshel","sequence":"additional","affiliation":[{"name":"Hematology Unit and Laboratories, Ziv Medical Center  Safed Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiza","family":"Akria","sequence":"additional","affiliation":[{"name":"Azrieli Faculty of Medicine Bar\u2010Ilan University  Safed Israel"},{"name":"Hematology Unit and Laboratories Galilee Medical Center  Naharia Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ety","family":"Shaoul","sequence":"additional","affiliation":[{"name":"Azrieli Faculty of Medicine Bar\u2010Ilan University  Safed Israel"},{"name":"Hematology Unit and Laboratories Galilee Medical Center  Naharia Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Netanel","family":"Horowitz","sequence":"additional","affiliation":[{"name":"The Ruth and Bruce Rappaport Faculty of Medicine, Department of Hematology and Bone Marrow Transplantation, Rambam Health Care Campus, Haifa Technion, Israel Institute of Technology  Haifa Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000501"},{"key":"e_1_2_5_3_1","first-page":"w14635","article-title":"Diagnosis and Treatment of Follicular Lymphoma: An Update","volume":"148","author":"Bargetzi M.","year":"2018","journal-title":"Swiss Medical Weekly"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/99.4.374"},{"key":"e_1_2_5_5_1","first-page":"5010","article-title":"Cellular DNA of human neoplastic B\u2010cells measured by flow cytometry","volume":"44","author":"Braylan R. 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