{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:31:02Z","timestamp":1780392662920,"version":"3.54.1"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T00:00:00Z","timestamp":1586131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Minimal residual disease (MRD) in chronic lymphocytic leukemia (CLL) has prognostic and predictive significance. One of the approaches to detect MRD by flow cytometry (FC) is the use of dry antibody reagents such as DuraClone\u00ae RE CLB (Beckman Coulter\u2014BC). The aim of this study was to evaluate the performance of the DuraClone\u00ae RE CLB in detecting MRD in CLL compared to liquid reagents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DuraClone\u00ae RE CLB is composed by CD81FITC, ROR1PE, CD79bPC5.5, CD19PC7, CD5APC, CD43APCA750, CD20PB, and CD45KrO. For the liquid reagent assay, we used CD43FITC, ROR1PE, CD3ECD, CD5PC5.5, CD20PC7, CD79bAPC, CD19APC750, CD81 APCH7, and CD45KrO. The liquid and dry tubes were used to detect 20 MRD\u2010positive CLL samples. The samples were analyzed using Radar Plots Kaluza Software (BC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The statistical correlation between the liquid and dry reagents was acceptable (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> =\u2009.9583) and no discrepancy was observed in MRD percentages. The average of the total number of acquired events in DuraClone\u00ae RE CLB was 758.583 (362.632\u20132.290.387), which allowed accurate sensitivity for the FC assay. The lowest MRD frequency detected by DuraClone\u00ae RE CLB was 0.01%, corresponding to a cluster with 106 events in a total of 737.030. The radar plots allowed the discrimination between normal B\u2010cell population and CLL cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The DuraClone\u00ae RE CLB method allowed the accurate detection of MRD in clinical and interlaboratorial CLL samples, thereby supporting the use of this method to potentially increase productivity, reduce pipetting\u2010associated errors and cost, and allow better standardization.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21875","type":"journal-article","created":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T16:10:47Z","timestamp":1586189447000},"page":"529-535","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Performance of eight\u2010color dry antibody reagent in the detection of minimal residual disease in chronic lymphocytic leukemia samples"],"prefix":"10.1002","volume":"98","author":[{"given":"Laiz","family":"Bento","sequence":"first","affiliation":[{"name":"Flow Cytometry Clinical Laboratory Hospital Isarelita Albert Einstein  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Correia","sequence":"additional","affiliation":[{"name":"Flow Cytometry Clinical Laboratory Hospital Isarelita Albert Einstein  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Flow Cytometry Clinical Laboratory Hospital Isarelita Albert Einstein  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andressa","family":"Vaz","sequence":"additional","affiliation":[{"name":"Flow Cytometry Clinical Laboratory Hospital Isarelita Albert Einstein  S\u00e3o Paulo 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