{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:38:22Z","timestamp":1790318302289,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T00:00:00Z","timestamp":1587513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A peripheral blood flow cytometric assay for S\u00e9zary syndrome (SS) or circulating mycosis fungoides (MF) cells must be able to reliably identify, characterize, and enumerate T\u2010cells with an immunophenotype that differs from non\u2010neoplastic T\u2010cells. Although it is also important to distinguish SS and MF from other subtypes of T\u2010cell neoplasm, this usually requires information in addition to the immunophenotype, such as clinical and morphologic features. This article outlines the approach recommended by an international group with experience and expertise in this area. The following key points are discussed: (a) At a minimum, a flow cytometric assay for SS and MF should include the following six antibodies: CD3, CD4, CD7, CD8, CD26, and CD45. (b) An analysis template must reliably detect abnormal T\u2010cells, even when they lack staining for CD3 or CD45, or demonstrate a phenotype that is not characteristic of normal T\u2010cells. (c) Gating strategies to identify abnormal T\u2010cells should be based on the identification of subsets with distinctly homogenous immunophenotypic properties that are different from those expected for normal T\u2010cells. (d) The blood concentration of abnormal cells, based on any immunophenotypic abnormalities indicative of MF or SS, should be calculated by either direct enumeration or a dual\u2010platform method, and reported.<\/jats:p>","DOI":"10.1002\/cyto.b.21878","type":"journal-article","created":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T10:56:20Z","timestamp":1587552980000},"page":"142-155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Flow cytometric evaluation of peripheral blood for suspected S\u00e9zary syndrome or mycosis fungoides: International guidelines for assay characteristics"],"prefix":"10.1002","volume":"100","author":[{"given":"Pedro","family":"Horna","sequence":"first","affiliation":[{"name":"Division of Hematopathology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa A.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristy L.","family":"Wolniak","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology Northwestern University Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherina","family":"Psarra","sequence":"additional","affiliation":[{"name":"Department of Immunology and Histocompatibility \u201cEvangelismos\u201d Hospital  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Almeida","sequence":"additional","affiliation":[{"name":"Cancer Research Center (IBMCC\u2010CSIC\/USAL\u2010IBSAL), Cytometry Service (NUCLEUS) and Department of Medicine, IBSAL and CIBERONC University of Salamanca  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea J.","family":"Illingworth","sequence":"additional","affiliation":[{"name":"Dahl\u2010Chase Diagnostic Services  Bangor Maine USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrika","family":"Johansson","sequence":"additional","affiliation":[{"name":"SI\u2010HMDS University Hospitals Bristol and Weston NHS Foundation Trust  Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1743-3454","authenticated-orcid":false,"given":"Fiona E.","family":"Craig","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic Arizona  Phoenix Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Torres","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Yale University School of Medicine  New Haven Connecticut USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.27.7665"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21020"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20395"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04014.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19960615)26:2<137::AID-CYTO7>3.0.CO;2-D"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.01.038"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20001215)42:6<327::AID-CYTO1000>3.0.CO;2-F"},{"issue":"16","key":"e_1_2_5_9_1","first-page":"2115","article-title":"Single\u2010cell heterogeneity in S\u00e9zary syndrome","volume":"2","author":"Buus T. 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