{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T20:53:09Z","timestamp":1773089589902,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T00:00:00Z","timestamp":1586304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We analyzed expression profiles of immune checkpoint receptors on T cell subsets and ligands on leukemic blasts in patients with B\u2010lymphoblastic leukemia (B\u2010ALL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Total 149 bone marrow (BM) samples obtained from 65 B\u2010ALL patients with four different clinical status (41 at diagnosis, 54 in complete remission [CR], 34 in persistence, and 20 in relapse), and 32 BM control samples were prospectively enrolled. Expression of immune checkpoint receptor (programmed cell death protein\u20101 [PD\u20101]) on T cell subsets and ligands (PD\u2010L1, PD\u2010L2) on leukemic blasts was evaluated by flow cytometry, and was compared between patient subgroups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Relapsed patients demonstrated highest PD\u20101 expression proportion and intensity on CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup> T cells with statistical significance when compared to patients in persistence\/CR\/BM controls (<jats:italic>p<\/jats:italic> = .027\/&lt;.001\/&lt;.001 and .012\/.001\/&lt;.001, respectively). Newly diagnosed patients showed significantly lower PD\u20101 expression proportion on CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup> T cells than relapsed patients (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001), but their intensity was not significantly different. Relapsed patients showed significantly higher PD\u20101 expression proportion and intensity on CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells than patients in CR\/BM controls (<jats:italic>p<\/jats:italic> = .022\/.045 and .049\/.005, respectively), but PD\u20101 expression status on them were not significantly different between relapsed and newly diagnosed patients. PD\u2010L1\/L2 expression on leukemic blasts was not significantly different between patient subgroups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In BM aspirates from B\u2010ALL patients, PD\u20101 expression on T\u2010cell subsets is increased at diagnosis, and to a greater extent, at relapse. These data suggest the potential usefulness of PD\u20101 blockade in the treatment of B\u2010ALL, particularly at relapse.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21879","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T17:28:01Z","timestamp":1586366881000},"page":"336-347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Increased expression of immune checkpoint programmed cell death protein\u20101 (<scp>PD<\/scp>\u20101) on T cell subsets of bone marrow aspirates in patients with <scp>B\u2010Lymphoblastic<\/scp> leukemia, especially in relapse and at diagnosis"],"prefix":"10.1002","volume":"98","author":[{"given":"Sang Hyuk","family":"Park","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine, Ulsan University Hospital  Ulsan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunkyoung","family":"You","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Inje University College of Medicine, Busan Baik Hospital  Busan South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan\u2010Jeoung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010Uk","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seongsoo","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Ulsan College of Medicine and Asan Medical Center  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho\u2010Joon","family":"Im","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Ulsan College of Medicine and Asan Medical Center  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong\u2010Jin","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Ulsan College of Medicine and Asan Medical Center  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han\u2010Seung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Hematology University of Ulsan College of Medicine and Asan Medical Center  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung\u2010Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Hematology University of Ulsan College of Medicine and 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