{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:43:51Z","timestamp":1792183431073,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T00:00:00Z","timestamp":1587772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Flow cytometric detection of T\u2010cell clonality is challenging. The current available methodology for T\u2010cell receptor (TCR) V\u03b2 repertoire evaluation is a complex assay and has limited sensitivity especially for detecting low levels of disease. Therefore, there is an unmet need for a reliable, simple, and rapid assay to identify T\u2010cell clonality. The rearrangement of the TCRB gene involves the random and mutually exclusive expression of one of two constant \u03b2 chain genes (TRBC1 and TRBC2), analogous to the kappa and lambda gene utilization by B cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Here, we used a single TRBC1 antibody, in conjunction with other T\u2010cell associated markers, to detect T\u2010cell clonality in tissue biopsies and body fluids. A total of 143 tissue\/body fluid specimens from 46 patients with a definitive diagnosis of a T\u2010cell neoplasm and 97 patients with no T\u2010cell malignancy were analyzed with a cocktail of monoclonal antibodies including CD2\/CD3\/CD4\/CD5\/CD7\/CD8\/CD45\/TCR\u03b3\u03b4\/TRBC1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We examined TRBC1 expression on neoplastic T\u2010cell populations identified based on their immunophenotypic aberrancies, and monotypic TRBC1 expression was identified in all 46 known T\u2010cell lymphoma cases. We applied a similar gating strategy to the 97 cases without T\u2010cell neoplasms, and arbitrarily dissected T\u2010cell populations into immunophenotypically distinct subsets; in this group, we found that all cases revealed an expected polytypic TRBC1 expression in all subsets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Single TRBC1 antibody detection of T\u2010cell clonality by flow cytometry is a simple, rapid, and robust assay that could be routinely utilized in flow cytometric laboratories.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cyto.b.21881","type":"journal-article","created":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T11:19:55Z","timestamp":1587813595000},"page":"361-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Flow cytometric evaluation of\n                    <scp>TRBC1<\/scp>\n                    expression in tissue specimens and body fluids is a novel and specific method for assessment of\n                    <scp>T\u2010cell<\/scp>\n                    clonality and diagnosis of\n                    <scp>T\u2010cell<\/scp>\n                    neoplasms"],"prefix":"10.1002","volume":"100","author":[{"given":"Holly","family":"Berg","sequence":"first","affiliation":[{"name":"Division of Hematopathology, Department of Pathology and Laboratory Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory E.","family":"Otteson","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology and Laboratory Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Corley","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology and Laboratory Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Shi","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology and Laboratory Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Horna","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology and Laboratory Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan","family":"Jevremovic","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology and Laboratory Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5783-4485","authenticated-orcid":false,"given":"Horatiu","family":"Olteanu","sequence":"additional","affiliation":[{"name":"Division of Hematopathology, Department of Pathology and Laboratory Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPPIBH3I1VRWXQ"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21020"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404481"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20107"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/QF6N-VAQW-N74H-4JE2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21768"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1431"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.03.009"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.246"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4444"},{"key":"e_1_2_6_12_1","first-page":"345","volume-title":"WHO Classification of tumours of haematopoietic and lymphoid tissues","author":"Mature T","year":"2017"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1309\/25E9Y7RRAY84HTAT"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1309\/3A32DTVMH640M2QA"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04201.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.path.2013.08.009"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqy173"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2008.01109.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-99-119564"},{"key":"e_1_2_6_20_1","first-page":"119","volume-title":"Flow cytometry in the assessment of hematologic disroders. neoplastic hematopathology","author":"Reichard K. 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