{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T17:24:14Z","timestamp":1783617854062,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T00:00:00Z","timestamp":1591228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There are no immunophenotypic guidelines for the investigation of <jats:italic>MYC<\/jats:italic>\u2010rearranged lymphomas. We aimed to identify simple immunophenotypic features that would help to differentiate between <jats:italic>MYC<\/jats:italic>\u2010rearranged lymphomas and guide cytogenetic analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We reviewed diagnostic samples from patients diagnosed with Burkitt lymphoma (BL), double\u2010hit lymphoma (DHL), <jats:italic>MYC<\/jats:italic>\u2010rearranged diffuse large B\u2010cell lymphoma (MYC\u2010DLBCL), and standard (non\u2010<jats:italic>MYC<\/jats:italic>\u2010rearranged) DLBCL over the last decade in our Institution. Using flow cytometry (with antibodies CD20, CD10, CD38, bcl\u20102, Ki\u201067, FMC\u20107, CD43, CD27, CD79b, CD23, and CD22) we determined antigen% expression and median\u2010fluorescence intensity ratios (MFIR). The forward scatter (FS) and side scatter (SS) characteristics of tumor B\u2010cells were compared with normal T\u2010cells (B\/T ratios) for patients with <jats:italic>MYC<\/jats:italic>\u2010rearranged lymphomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 51 patients of whom 14 had BL, 10 had DHL (6 <jats:italic>MYC<\/jats:italic>+\/<jats:italic>BCL2<\/jats:italic>+; 4 <jats:italic>MYC<\/jats:italic>+\/<jats:italic>BCL<\/jats:italic>6+), 8 MYC\u2010DLBCL, and 19 standard DLBCL. The significant differences (<jats:italic>p<\/jats:italic> &lt;.05) were: higher CD38% in BL than standard DLBCL; higher CD10% in BL and DHL versus MYC\u2010DLBCL and standard DLBCL; higher CD10MFIR in BL than MYC\u2010DLBCL and standard DLBCL; higher Ki\u201067% in BL than DHL and MYC\u2010DLBCL; higher bcl\u20102% in DHL than BL; higher FMC\u20107% in BL than MYC\u2010DLBCL and standard DLBCL; and lower SS (B\/T) ratio in DHL than MYC\u2010DLBCL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The combination of CD38%\u2009&gt;\u200990, CD10%\u2009&gt;\u200980, CD10MFIR\u2009&gt;\u200910, bcl\u20102%\u2009&lt;\u200930, and Ki\u201067%\u2009&gt;\u200970 was characteristic of BL. \u201cDeviation\u201d from these cut\u2010offs should raise suspicion for DHL and, therefore, <jats:italic>BCL2<\/jats:italic> and\/or <jats:italic>BCL6<\/jats:italic> FISH is required. We also found that a diagnosis of DHL rather than of MYC\u2010DLBCL was significantly associated with CD10%\u2009&gt;\u200960, Ki\u201067%\u2009&gt;\u200950, and SS (B\/T) &lt;1.5.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21887","type":"journal-article","created":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T18:38:44Z","timestamp":1591295924000},"page":"412-420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Contribution of immunophenotype to the investigation and differential diagnosis of Burkitt lymphoma, double\u2010hit high\u2010grade B\u2010cell lymphoma, and single\u2010hit <scp><i>MYC<\/i><\/scp>\u2010rearranged diffuse large B\u2010cell lymphoma"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9759-0048","authenticated-orcid":false,"given":"Nikolaos J.","family":"Tsagarakis","sequence":"first","affiliation":[{"name":"Department of Laboratory Hematology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanos I.","family":"Papadhimitriou","sequence":"additional","affiliation":[{"name":"Department of Laboratory Hematology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitris","family":"Pavlidis","sequence":"additional","affiliation":[{"name":"Department of Laboratory Hematology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos","family":"Liapis","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Gortzolidis","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis V.","family":"Kostopoulos","sequence":"additional","affiliation":[{"name":"Department of Laboratory Hematology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodoros","family":"Marinakis","sequence":"additional","affiliation":[{"name":"Department of Clinical Hematology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Paterakis","sequence":"additional","affiliation":[{"name":"Flow Cytometry Laboratory, Department of Immunology Athens Regional General Hospital Georgios Gennimatas  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2016.07.005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2015-203002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21770"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.091827"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-09-297879"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-657379"},{"issue":"12","key":"e_1_2_8_8_1","first-page":"920","article-title":"Diagnostic and therapeutic values of interphase fluorescence in situ hybridization in B\u2010cell lymphomas: A clinicopathologic analysis of 604 cases","volume":"47","author":"Chen M.","year":"2018","journal-title":"Chinese Journal of Pathology"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-03-836361"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21227"},{"key":"e_1_2_8_11_1","first-page":"140","article-title":"Diffuse large B\u2010cell lymphoma with MYC gene rearrangements: Current perspective on treatment of diffuse large B\u2010cell lymphoma with MYC gene rearrangements; case series and review of the literature","volume":"55","author":"Jonge A. 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