{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:26:42Z","timestamp":1780392402356,"version":"3.54.1"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Within the hematopoietic compartment, fibromodulin (FMOD) is almost exclusively expressed in chronic lymphocytic leukemia (CLL) lymphocytes. We set out to determine whether FMOD could be of help in diagnosing borderline lymphoproliferative disorders (LPD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We established 3 flow cytometry\u2010defined groups (CLL [<jats:italic>n<\/jats:italic> =\u200965], borderline LPD [<jats:italic>n<\/jats:italic> =\u200928], broadly defined as those with CLLflow score between 35 and \u221220 or discordant CD43 and CLLflow, and non\u2010CLL LPD [<jats:italic>n<\/jats:italic> =\u200940]). FMOD expression levels were determined by standard RT\u2010PCR in whole\u2010blood samples. Patients were included regardless of lymphocyte count but with tumor burden \u226540%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>FMOD expression levels distinguished between CLL (median 98.5, interquartile range [IQR] 37.8\u2013195.1) and non\u2010CLL LPD (median 0.012, IQR 0.003\u20130.033) with a sensitivity and specificity of 1. Most borderline LPDs were CD5\/CD23\/CD200\u2010positive with no loss of B\u2010cell antigens and negative or partial expression of CD43. 16\/22 patients with available cytogenetic analysis showed trisomy 12. In 25\/28 (89%) of these patients, FMOD expression levels fell between CLL and non\u2010CLL (median 3.58, IQR 1.06\u20136.21).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This study could suggest that borderline LPDs may constitute a distinct group laying in the biological spectrum of chronic leukemic LPDs. Future studies will have to confirm these results with other biological data. Quantification of FMOD can potentially be of help in the diagnosis of phenotypically complex LPDs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21890","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T14:57:08Z","timestamp":1591973828000},"page":"421-428","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<scp>FMOD<\/scp> expression in whole blood aids in distinguishing between chronic lymphocytic leukemia and other leukemic lymphoproliferative disorders. A pilot study"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0587-591X","authenticated-orcid":false,"given":"Marc","family":"Sorigue","sequence":"first","affiliation":[{"name":"Hematology Laboratory ICO\u2010Hospital Germans Trias i Pujol, Institut de Recerca Josep Carreras, Universitat Aut\u00f2noma de Barcelona  Badalona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Junca","sequence":"additional","affiliation":[{"name":"Hematology Laboratory ICO\u2010Hospital Germans Trias i Pujol, Institut de Recerca Josep Carreras, Universitat Aut\u00f2noma de Barcelona  Badalona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Ferra","sequence":"additional","affiliation":[{"name":"Department of Clinical Haematology ICO\u2010Hospital Germans Trias i Pujol, Institut de Recerca Josep Carreras, Universitat Aut\u00f2noma de Barcelona  Badalona 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