{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T12:50:17Z","timestamp":1779281417160,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T00:00:00Z","timestamp":1594771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Blood involvement by mycosis fungoides (MF)\/S\u00e9zary syndrome (SS) influences prognosis and therapeutic decisions. MF\/SS blood stage is currently determined by absolute CD4\u2009+\u2009CD26\u2212 or CD4\u2009+\u2009CD7\u2010cell counts, which quantification method may overestimate MF\/SS by including CD26\u2212 or CD7\u2212 normal CD4+ T\u2010cells, or underestimate disease burden when MF\/SS cells show incomplete loss of CD26 and\/or CD7. Recently, through the standardization effort led by the International Clinical Cytometry Society (ICCS), recommendation was made to quantify MF\/SS by enumerating immunophenotypically aberrant CD4+ T\u2010cells, rather than CD26\u2212 or CD7\u2212 in isolation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared these two quantitation methods in 309 MF\/SS patients who had blood samples analyzed by flow cytometry immunophenotyping (FCI) over a 1\u2010year period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using the European Organization of Research and Treatment of Cancer (EORTC)\/International Society for Cutaneous Lymphomas (ISCL) criteria, 221 (71.5%) patients had a blood stage corresponding to B0, 57 (18.4%) to B1, and 31 (10%) to B2. By FCI analysis, a total of 62 patients (20.0%) were found positive for MF\/SS. Among EORTC B0 patients, 11\/221 (5%) were positive by FCI (false negatives), and among EORTC Stage B1 patients, 35\/57 (61%) were negative by FCI (false positives). Regarding patients positive for MF\/SS cells by FCI, there was an overall excellent correlation (<jats:italic>r<\/jats:italic> =\u2009.999, <jats:italic>p<\/jats:italic> &lt;\u2009.001) between the EORTC\/ISCL method and FCI method; however, four (6.5%) patients would have an altered B stage between B0 and B1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The MF\/SS cell quantification method using immunophenotypic aberrancies, as recommended by the ICCS, allows to distinguish MF\/SS cells from background benign T\u2010cells and enables for more accurate staging, especially among patients currently being considered to have B0 and B1 stage diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21933","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T13:20:49Z","timestamp":1594819249000},"page":"183-191","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Determination of immunophenotypic aberrancies provides better assessment of peripheral blood involvement by mycosis fungoides\/S\u00e9zary syndrome than quantification of <scp>CD26<\/scp>\u2212 or <scp>CD7<\/scp>\u2212 <scp>CD4<\/scp>+ T\u2010cells"],"prefix":"10.1002","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9983-9735","authenticated-orcid":false,"given":"Kirill A.","family":"Lyapichev","sequence":"first","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismael","family":"Bah","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Auris","family":"Huen","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madeleine","family":"Duvic","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark J.","family":"Routbort","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey L.","family":"Jorgensen","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Jeffrey","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Vega","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1743-3454","authenticated-orcid":false,"given":"Fiona E.","family":"Craig","sequence":"additional","affiliation":[{"name":"Division of Hematopathology Mayo Clinic Arizona  Phoenix Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sa A.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(88)70277-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.27.7665"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04014.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.01.038"},{"issue":"6","key":"e_1_2_6_6_1","first-page":"859","article-title":"Abnormalities of circulating T\u2010cell subpopulations in patients with cutaneous T\u2010cell lymphoma: Cutaneous lymphocyte\u2010associated antigen expression on T cells correlates with extent of disease","volume":"7","author":"Borowitz M. 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