{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:22:40Z","timestamp":1790392960909,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T00:00:00Z","timestamp":1595808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The Matutes score (MS) was proposed to differentiate chronic lymphocytic leukemia (CLL) from other B\u2010cell non\u2010Hodgkin lymphomas (B\u2010NHLs). However, ambiguous immunophenotypes are common and remain a diagnostic challenge. Therefore, we evaluated the diagnostic benefit of measuring CD200 and CD43 expression together with the standard MS antigens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>138 lymphoma patient samples and a validation cohort of 138 additive samples were classified according to the standard MS and further assigned with one or two additional points, for high CD200 and\/or CD43 expression levels. The \u201c<jats:italic>classical<\/jats:italic>\u201d MS and the \u201c<jats:italic>Matutes score\u2010extended<\/jats:italic>\u201d (MS\u2010e) were categorized as high (4\u20105\/6\u20107), intermediate (2\u20103\/4\u20105), and low (0\u20101\/0\u20103). Samples were reclassified into the MS\u2010e with focus on ambiguous cases with an intermediate \u201c<jats:italic>classical<\/jats:italic>\u201d MS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 35 of 138 (25.4%) patient samples were assigned to the intermediate MS group and confirmed by histopathological reports as CLL (14\/40.0%) and B\u2010NHLs other than CLL (21\/60%). MS\u2010e analysis identified 13 of 14 (92.9%) of CLL cases (MS\u2010e 4\u20135) and 18\/21 (85.7%) non\u2010CLL cases (MS\u2010e\u2009\u2264\u20093) correctly. Overall, the sensitivity of the CLL diagnosis was significantly increased by application of MS\u2010e compared to the \u201c<jats:italic>classical<\/jats:italic>\u201d MS (98.8% vs. 82.7%; <jats:italic>p<\/jats:italic> = 0.0009), while specificity of both methods was almost equal (94.7% vs. 98.3%; <jats:italic>p<\/jats:italic> = 0.4795). Of note, sole measurement of CD43 and CD200 on B\u2010cells sufficiently differentiated CLL from non\u2010CLL with a test accuracy superior to the \u201c<jats:italic>classical<\/jats:italic>\u201d MS (F1 score 96.2 vs. 93.6).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CD200 and CD43 have a high informative value in diagnostic immunophenotyping and facilitate the separation of CLL from other B\u2010NHLs particularly in ambiguous cases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21936","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T16:40:16Z","timestamp":1595868016000},"page":"476-482","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Determination of <scp>CD43<\/scp> and <scp>CD200<\/scp> surface expression improves accuracy of B\u2010cell lymphoma immunophenotyping"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2820-1501","authenticated-orcid":false,"given":"Joerg","family":"Hoffmann","sequence":"first","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marissa","family":"Rother","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Innere Medizin II  Hildesheim Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael C.","family":"Thrun","sequence":"additional","affiliation":[{"name":"Databionics, Mathematics and Computer Science Philipps University Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Wilhelm","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Gruen","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Niebergall","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Meissauer","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Neubauer","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Brendel","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Immunology, Philipps University Marburg University Hospital Giessen and Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP59UORCYZEVQO"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V71.5.1226.1226"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07644.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"12779","DOI":"10.1016\/S0021-9258(18)67161-3","article-title":"Isolation and characterization of leukosialin, a major sialoglycoprotein on human leukocytes","volume":"261","author":"Carlsson S. 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