{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:03:32Z","timestamp":1789689812521,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytometry Part B Clinical"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Myeloid neoplasm with blasts showing mast cell (MC)\u2010differentiation and MC\u2010component less than 10% of all nucleated cells but not fulfilling the criteria for systemic mastocytosis with associated hematological neoplasm (SM\u2010AHN) or myelomastocytic leukemia (MML) has not been described in the literature. Herein, we report a study of diverse myeloid malignancies with blasts showing MC\u2010differentiation but not meeting the criteria for SM\u2010AHN or MML. We also evaluated the utility of flow\u2010cytometric immunophenotyping (FCI) in the characterization of immature\u2010MCs (iMCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We identified nine patients of myeloid neoplasms and studied their morphological, FCI, immunohistochemistry, cytogenetic and molecular characteristics. We also compared the immunophenotypic features of MCs from patient samples with control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study included patients with newly\u2010diagnosed acute myeloid leukemia (<jats:italic>n<\/jats:italic>\u00a0=\u00a04), chronic myelomonocytic leukemia (<jats:italic>n<\/jats:italic>\u00a0=\u00a01), and chronic myeloid leukemia on follow\u2010up (<jats:italic>n<\/jats:italic>\u00a0=\u00a04) showing MC differentiation in leukemic\u2010blasts. These patients had mildly increased MCs (range, 0.5%\u20133%) in bone\u2010marrow morphology, including immature\u2010forms and did not meet the criteria for either SM\u2010AHN or MML. On FCI, iMCs were positive for bright\u2010CD117, heterogeneous\u2010CD34, dim\u2010to\u2010negative\u2010HLADR, and moderate\u2010CD203c expression. Expression\u2010levels of CD123 and CD38 were higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) but CD33 and CD45 were lower in iMCs compared to mature\u2010MC from control samples (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.019 and <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0037).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We reported a rare finding of MC differentiation of leukemic blasts in diverse myeloid neoplasms and proposed it as a potential pre\u2010myelomastocytic leukemia condition. We described the distinct immunophenotypic signature of immature\u2010MCs using commonly used markers and highlighted the utility of FCI for the diagnosis of this entity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.b.21938","type":"journal-article","created":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T14:50:40Z","timestamp":1596293440000},"page":"331-344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Mast cell differentiation of leukemic blasts in diverse myeloid neoplasms: A potential pre\u2010myelomastocytic leukemia condition"],"prefix":"10.1002","volume":"100","author":[{"given":"Devasis","family":"Panda","sequence":"first","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6505-7898","authenticated-orcid":false,"given":"Gaurav","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Twinkle","family":"Khanka","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sitaram","family":"Ghogale","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajamanam","family":"Badrinath","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilesh","family":"Deshpande","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohan","family":"Sardana","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anumeha","family":"Chaturvedi","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sweta","family":"Rajpal","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhanalaxmi","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Cancer Cytogenetics, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil V.","family":"Patkar","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumeet","family":"Gujral","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"},{"name":"Department of Pathology Tata Memorial Center  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Papagudi G.","family":"Subramanian","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9030-0415","authenticated-orcid":false,"given":"Prashant R.","family":"Tembhare","sequence":"additional","affiliation":[{"name":"Hematopathology Laboratory, ACTREC, Tata Memorial Center HBNI University  Navi Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21713"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612822666160928121857"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21399"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2014.01.018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/1998\/341340"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-11-189605"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v31.i6.30"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2012.01.007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.5858\/2008-132-813-ISOBBM"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.014860"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000305552"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(01)00021-2"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12221"},{"key":"e_1_2_8_16_1","first-page":"1493","article-title":"Flow cytometric analysis of mast cells from normal and pathological human bone marrow samples: Identification and enumeration","volume":"149","author":"Orfao A.","year":"1996","journal-title":"The American Journal of Pathology"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.advms.2018.08.013"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385493-3.00014-0"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2014.01.008"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.07.016"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.224"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2677"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-1064"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.293"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.5RU0614-296R"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-09-731893"},{"key":"e_1_2_8_28_1","first-page":"1261","article-title":"Advances in the classification and treatment of Mastocytosis: Current status and outlook toward the future","volume":"77","author":"Valent P.","year":"2017","journal-title":"Blood"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(01)00038-8"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.thj.6200164"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2010.550072"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdu047"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.12.4322.bloodjournal84124322"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(01)00040-6"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2018.04.014"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199905)61:1<66::AID-AJH12>3.0.CO;2-3"}],"container-title":["Cytometry Part B: Clinical Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.b.21938","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21938","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cyto.b.21938","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.b.21938","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T01:24:26Z","timestamp":1693617866000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.b.21938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1002\/cyto.b.21938"],"URL":"https:\/\/doi.org\/10.1002\/cyto.b.21938","archive":["Portico"],"relation":{},"ISSN":["1552-4949","1552-4957"],"issn-type":[{"value":"1552-4949","type":"print"},{"value":"1552-4957","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"2020-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}